目的 研究原白頭翁素對(duì)銅綠假單胞菌（PA）的生長(zhǎng)及群體感應(yīng)系統(tǒng)（QS系統(tǒng)）調(diào)控的毒力因子表達(dá)的影響。方法 測(cè)定原白頭翁素對(duì)PA的最低抑菌濃度和生長(zhǎng)曲線，以40 μmol/L的呋喃酮C-30作為陽(yáng)性對(duì)照，測(cè)定原白頭翁素（40、80 μmol/L）對(duì)銅綠假單胞菌標(biāo)準(zhǔn)株P(guān)AO1生長(zhǎng)曲線的影響以及QS系統(tǒng)調(diào)控的毒力因子中胞外3種毒力因子（綠膿菌素、蛋白水解酶和彈性蛋白酶）表達(dá)量的影響。結(jié)果 在實(shí)驗(yàn)所采用的濃度下，原白頭翁素對(duì)銅綠假單胞菌的生長(zhǎng)未表現(xiàn)出明顯的抑制作用，但對(duì)PAO1的3種毒力因子均有抑制作用，并呈現(xiàn)出劑量相關(guān)性。結(jié)論 原白頭翁素抗感染的機(jī)制可能是作用于銅綠假單胞菌的QS系統(tǒng)，抑制毒力因子表達(dá)，從而降低該菌的毒力。

Objective To study the effect of protoanemonin on the expression of virulence factors regulated by quorum sensing (QS) system of Pseudomonas aeruginosa (PA). Methods The minimum inhibitory concentration and growth curve of protoanemonin on PA were determined. With furanone C-30 (40 μmol/L) as positive control, the effect of protoanemonin (40 and 80 μmol/L) on the growth of PAO1 was assessed, and the effects on the expression levels of the extracellular virulence factors (pyocyanin, protease, and elastase) regulated by QS system were also evaluated. Results Protoanemonin in concentration used in this study had no significant effect on the growth of PAO1, but it had the inhibitory effect on the three virulence factors of PAO1 with a dosage-dependent manner. Conclusion It is possible that protoanemonin acts on QS system of PA to inhibit the expression of virulence factors, in order to reduce the virulence of the bacteria.