目的 觀察消水腫膏對氫化可的松致腎陽虛大鼠腎功能改善的作用。方法 將大鼠隨機分為4組，正常組，模型組，消水腫膏高、低劑量組。正常組大鼠im生理鹽水1 mL/(kg·d)，其余大鼠均im氫化可的松25 mg/(kg·d)，給藥體積1 mL/(kg·d)，連續(xù)8 d。消水腫膏高、低劑量組分別連續(xù)ig消水腫膏42、21 g生藥/kg，2次/d，連續(xù)12 d，正常組與模型組大鼠ig相同體積純凈水。觀察各組大鼠體質(zhì)量變化；末次給藥1 h后，用4.5%戊巴比妥鈉麻醉大鼠，經(jīng)腹主動脈取血，測定血清醛固酮、肌酐、尿素；取尿液測定尿蛋白、尿Na+、K+濃度；取腎臟、脾臟稱量質(zhì)量。結(jié)果 給藥12 d，消水腫膏高、低劑量組大鼠體質(zhì)量較模型組有增加趨勢，但未見明顯統(tǒng)計學(xué)差異（P＞0.05）。消水腫膏高劑量組大鼠腎臟與脾臟質(zhì)量均明顯高于模型組（P＜0.05），血清肌酐水平顯著低于模型組（P＜0.05）。消水腫膏高劑量對尿蛋白、尿K+的影響與模型組比較無顯著差異（P＞0.05），有增加尿Na+排泄量的趨勢。結(jié)論 消水腫膏能夠?qū)箽浠傻乃伤碌哪I陽虛大鼠腎臟、脾臟質(zhì)量減輕，降低其血清肌酐，可增加尿Na＋的排泄量，對腎陽虛大鼠的腎功能具有明顯改善作用。

Objective To study the amelioration of Xiaoshuizhong Ointment (XO) on the renal function of kidney Yang deficiency rats induced by hydrocortisone. Methods Rats were randomly divided into four groups, including control, model, low-, and high-dose XO groups. The rats in the control group were im administered with physiological saline [1 mL/(kg·d)], others were im administered with hydrocortisone [25 mg/(kg·d)], and the volume of administration was 1 mL/(kg·d). The rats in all groups were consecutively adminstered for 8 d. The rats in low- and high-dose XO groups were ig administered with XO (21 and 42 g /kg), twice daily, for 12 d. The control and model groups were ig administered with pure water. The changes in the body weight of rats were observed. One hour after the last administration, the blood was obtained through abdominal aorta after the rats were anesthetized by 4.5% pentobarbital sodium, then the serum aldosterone, creatinine, and urea were evaluated, urine protein, concentration of Na+ and K+ in urinary were determinated, and the kidney and spleen of rats were weighed. Results After 12 d of administration, the body weight of rats in low- and high-dose XO groups showed a increasing trend compared with those in the model group without statistical significance (P＞0.05). The kidney and spleen weight of rats in high-dose XO group were significantly higher than those in the model group (P＜0.05). Serum creatinine of rats in high-dose XO group was significantly lower than that in the model group (P＜0.05). Urine protein and concentration of K+ in urinary had no significant difference between groups (P＞0.05), but high-dose XO could increase the excretion of urinary Na+. Conclusion XO could prevent kidney and spleen from weight losing of kidney Yang deficiency rats induced by hydrocortisone, reduce the serum creatinine, and increase Na+ excretion of urine.XO has obvious amelioration on the renal function of kidney Yang deficiency rats.

天津市衛(wèi)生局中醫(yī)、中西醫(yī)結(jié)合科研課題（07054）