目的 分離提取穿龍薯蕷內(nèi)生真菌，篩選能夠發(fā)酵提高穿山龍藥材中薯蕷皂苷元的菌種。方法 采用PDA培養(yǎng)基分離純化穿龍薯蕷內(nèi)生真菌，將內(nèi)生真菌與121 ℃濕熱滅菌后的穿山龍藥材粗粉在28 ℃條件下固體發(fā)酵培養(yǎng)15 d，以HPLC法測定發(fā)酵物中薯蕷皂苷元，并與穿山龍原藥材、滅菌空白藥材以及未經(jīng)滅菌藥材的發(fā)酵物進(jìn)行比較。結(jié)果 分離篩選的穿龍薯蕷內(nèi)生真菌C39能夠穩(wěn)定提高滅菌后穿山龍藥材中薯蕷皂苷元的水平，提高幅度達(dá)原藥材的70%～75%。結(jié)論 采用固體發(fā)酵的方法可以使穿龍薯蕷內(nèi)生真菌C39穩(wěn)定提高穿山龍藥材中薯蕷皂苷元。

Objective To isolate and screen the endophytes from Dioscorea nipponica for increasing the diosgenin content in the dried rhizome by fermentation. Methods The endophytes were isolated and purified from D. nipponica in PDA culture medium. The raw powder of Dioscoreae Nippponicae Rhizoma was sterilized by moist and heat condition at 121 ℃, then fermented with the endophytes at 28 ℃ for 15 d. The diosgenin contents in the fermentation products were analyzed by HPLC and further compared with those of dried raw rhizome, sterilized dried rhizome, and the unsterilized fermented rhizome. Results The endophyte C39 isolated from D. nipponica increased the diosgenin content in sterilized dried Dioscoreae Nippponicae Rhizoma steadily in 70%—75% compared with the raw rhizome. Conclusion Endophyte C39 could increase the diosgenin content in Dioscoreae Nippponicae Rhizoma by solide fermentation.

國家自然科學(xué)基金資助項(xiàng)目（81274009）；教育部博士點(diǎn)專項(xiàng)基金資助項(xiàng)目（博導(dǎo)類20112327110003）；黑龍江中醫(yī)藥大學(xué)教育部重點(diǎn)實(shí)驗(yàn)室開放基金資助項(xiàng)目（S201101）