目的 采用高效液相色譜法測定加味逍遙丸中甘草酸的方法。方法 色譜條件：Kromasil C18柱（250 mm×4.6 mm，5 μm）；流動相：乙腈–0.017 mol/L磷酸（36︰64）；體積流量：1 mL/min；柱溫：30 ℃；檢測波長：250 nm。結(jié)果 甘草酸在0.084～0.500 μg線性關(guān)系良好，r＝0.999 9，回收率為98.5%，RSD為0.80%（n＝6）。結(jié)論 本法準(zhǔn)確可靠，重現(xiàn)性好，可用于測定加味逍遙丸中甘草酸。

Objective To establish an HPLC method for the determination of glycyrrhizic acid in Jiawei Xiaoyao Pills. Methods The chromatographic separation was performed on Kromasil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-0.017 mol/L phosphoric acid (36∶64), the flow rate was 1 mL/min at 30 ℃, and the detection wavelength was 250 nm. Results The linearity for glycyrrhizic acid was in a range of 0.084—0.500 μg (r = 0.999 9), and the average recovery and RSD were 98.5% and 0.80%, respectively (n = 6). Conclusion The method is accurate, reliable, and reproducible, and could be used as a quality control method for the determination of glycyrrhizic acid in Jiawei Xiaoyao Pills.