目的 研究脹果甘草藥渣總黃酮和其指標性成分甘草查爾酮A的制備及其體外抗腫瘤活性。方法 利用大孔樹脂柱色譜、聚酰胺柱色譜等方法，制備甘草藥渣總黃酮，并結(jié)合色譜法和波譜法分離鑒定指標性甘草查爾酮A，應(yīng)用HPLC法測定了總黃酮中甘草查爾酮A。應(yīng)用A549、H1792、Calu-1 3種人癌細胞系，采用MTT法和流式細胞術(shù)法系統(tǒng)評價甘草藥渣總黃酮和甘草查爾酮A的體外抗腫瘤活性和作用機制。結(jié)果 制備得到甘草藥渣總黃酮，并從中分離鑒定了特征性指標性成分甘草查爾酮A，測定其在甘草藥渣總黃酮中質(zhì)量分數(shù)為7.41%。甘草藥渣總黃酮和甘草查爾酮A對A549、H1792、Calu-1人癌細胞系均具有顯著的抑制活性，可誘導(dǎo)腫瘤細胞凋亡。經(jīng)流式細胞術(shù)檢測，推測其作用機制為誘導(dǎo)腫瘤細胞凋亡。結(jié)論 甘草查爾酮A為脹果甘草藥渣總黃酮發(fā)揮體外抗腫瘤活性的有效成分。

Objective To prepare the total flavonoids and its characteristic constituent licochalcone A from the residues of Glycyrrhiza inflata, which were further assayed for the antitumor activities in vitro. Methods The total flavonoids and licochalcone A were purified by chromatography. The structure of licochalcone A was identified on the basis of spectral data. The concentration of licochalcone A was determined by HPLC analysis method. Using MTT and flow cytometry, the antitumor activities in vitro were tested against A549, H1972, and Calu-1 human cancer cell lines. Results The total flavonoids and licochalcone A were prepared respectively and the concentration of licochalcone A was determined as 7.41% in the total flavonoids. The total flavonoids and licochalcone A showed the significant antitumor activities against the above three cancer cell lines. Its possible mechanism was the induction of apoptosis. Conclusions Licochalcone A is the effective antitumor constituent of the total flavonoids from the residues of G. inflata.

新疆維吾爾自治區(qū)科技支撐計劃項目（201333102）；新疆維吾爾自治區(qū)衛(wèi)生廳青年科技人才專項科研項目（2012Y06）