目的 建立薄層色譜法定性鑒別舒眠片中柴胡、白芍，并建立HPLC-ELSD法測定舒眠片中酸棗仁皂苷A的方法。方法 Diamonsil C18色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相：乙腈–0.1%磷酸水溶液，梯度洗脫；體積流量：1 mL/min；進(jìn)樣量：10 μL；漂移管溫度：105 ℃；氣體流量：2.8 L/min。結(jié)果 薄層色譜法鑒別出柴胡、白芍，酸棗仁皂苷A在20～120 μg/mL呈良好的線性關(guān)系（r＝0.999 8），平均回收率為99.75%，RSD值為0.34%。結(jié)論 柴胡、白芍的薄層色譜定性鑒別，操作簡便，且陰性無干擾；酸棗仁皂苷A的HPLC-ELSD測定方法準(zhǔn)確、簡便、重復(fù)性好，可以作為舒眠片的質(zhì)量控制方法。

Objective To establish a quantitative method for the identification of Bupleuri Radix and Paeoniae Alba Radix in Shumian Tablets by TLC, and the determination of jujuboside A in Shumian Tablets by HPLC-ELSD. Methods Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile - 0.1% phosphoric acid water solution and the gradient elution was carried out. The flow rate was 1.0 mL/min with injection volume 10 μL. The temperature of column and drift tube was set at 105 ℃. The flow rate of gas maintained at 2.8 L/min. Results Bupleuri Radix and Paeoniae Alba Radix were identified by TLC. The linear ranges of jujuboside A was 20 — 120 μg/mL (r=0.999 8). The average recovery was 99.75% (RSD=0.34%). Conclusion The method for the identification of Bupleuri Radix and Paeoniae Alb Radix in Shumian Tablets by TLC is simple and negative sample has no interference. The method for the determination of jujuboside A is accurate, simple, and reproducible. The method can be used for the quality control of Shumian Tablets.