目的 建立乳癰消痛片中熊果酸的HPLC測定方法。方法 采用高效液相色譜法，Kromasil C18色譜柱（250 mm×4.6 mm，5 μm）；流動相：甲醇–2%醋酸水溶液（90∶10）；體積流量：1.0 mL/min；柱溫：35 ℃；檢測波長：210 nm。結(jié)果 熊果酸在0.506～4.554 μg線性關(guān)系良好（r=1.000），平均加樣回收率為99.95%，RSD值為0.98%（n=9）。結(jié)論 該方法靈敏度高、準確、快速、專屬性強、重復性好，可用于乳癰消痛片中熊果酸的質(zhì)量控制。

Objective To establish an HPLC method for determination of ursolic acid in Ruyong Xiaotong Tablet. Methods Ursolic acid was determined on Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with methanol-2% acetic acid (90∶10) as mobile phase. The flow rate was 1.0 mL/min. The column temperature was 35 ℃, and the detection wavelength was 210 nm. Results The ursolic acid showed a good linear relationship in the range of 0.506—4.554 μg (r = 1.000). The average recovery of ursolic acid was 99.95% with RSD of 0.98% (n=9). Conclusion This method is accurate, fast, reliable, reproducible, and suitable for the determination of ursolic acid in Ruyong Xiaotong Tablet.