目的 研究茯苓多糖對Lewis肺癌小鼠自發(fā)肺轉(zhuǎn)移的影響，并探討其作用機制。方法 C57BL/6小鼠右腋皮下接種Lewis肺癌細胞，分為茯苓多糖高（0.50 mg/只）、低（0.33 mg/只）劑量組，順鉑組（順鉑0.04 mg/只），模型組（生理鹽水），接種瘤細胞后第2天開始尾iv給藥，隔天一次。觀察小鼠一般狀態(tài)，造模后第7天開始，隔天測量腫瘤體積。造模后21 d取肺，計數(shù)肺表面轉(zhuǎn)移灶個數(shù)，HE染色檢測肺微小轉(zhuǎn)移灶個數(shù)，檢測外周血白細胞數(shù)量及脾質(zhì)量、脾指數(shù)。結(jié)果 茯苓多糖高、低劑量對實體瘤無明顯抑制，茯苓多糖高劑量可減少肺表面轉(zhuǎn)移灶個數(shù)，增加白細胞CD11bmRNA表達，不影響外周血白細胞數(shù)、脾質(zhì)量及脾指數(shù)，一般狀況較好；茯苓多糖低劑量對肺表面轉(zhuǎn)移灶個數(shù)、外周血白細胞數(shù)、脾質(zhì)量及脾指數(shù)無明顯影響，可增加白細胞CD11b、CD18mRNA表達，一般狀況較好。結(jié)論 茯苓多糖對Lewis肺癌小鼠實體瘤無明顯抑制作用，但能夠抑制其自發(fā)肺轉(zhuǎn)移，對外周血白細胞數(shù)量、脾質(zhì)量及脾指數(shù)無明顯影響，可增加外周血白細胞CD11b、CD18mRNA表達，活化外周血白細胞可能是茯苓多糖抑制腫瘤轉(zhuǎn)移的機制之一。

Objective To investigate the effects and mechanism of pachymaran on spontaneous lung metastasis in Lewis lung cancer mice model. Methods C57BL/6 mice were sc injected with Lewis lung cells in the right axilla, and the mice were divided into four groups with ten mice each, such as model (physiological saline), cisplatin (0.04 mg), and pachymaran high- and low-dose (0.50 and 0.33 mg) groups, and from the day 2 of model establishment, the drug was given through tail vein every 2 d. The tumor volume was measured from 7 d to 21 d every two days. The number of metastasis on the surface of the lung was calculated after 21 d. The number of micrometastases in lung was calculated by HE staining. The numbers of peripheral blood leukocytes, spleen weight, and spleen index were also detected. Results The low- and high-dose pachymaran had no effect on the growth of the tumor. The high-dose pachymaran could reduce the number of lung surface metastases and increase the expression of CD11b mRNA in peripheral white blood cells. It had no effect on the number of the peripheral blood leukocyte, spleen weight, and spleen index. The low-dose pachymaran had no effect on the number of lung surface metastases, the number of the peripheral blood leukocyte, spleen weight, and spleen index. But it could increase the expression of CD11b mRNA and CD18 mRNA in peripheral white blood cells. Conclusion Pachymaran has no obvious inhibitory effect on Lewis lung cancer solid tumor in mice, but could inhibit the spontaneous lung metastasis. It has no significant effect on peripheral blood white cell count, spleen weight, and spleen index, but it may increase the expression of CD11b and CD18mRNA of peripheral white blood cells. It is suggested that the activation of peripheral white blood cells could be involved in the mechanism of pachyman inhibitory mechanism of tumor metastasis.

天津市應(yīng)用基礎(chǔ)及前沿技術(shù)研究計劃項目（10JCZDJC21300）