目的 研究熱毒寧注射液及其組分對(duì)流感病毒神經(jīng)氨酸酶（NA）抑制活性。方法 通過(guò)測(cè)定流感病毒NA的熒光強(qiáng)度值來(lái)確定熱毒寧注射液及其組分對(duì)流感病毒NA的抑制活性。結(jié)果 熱毒寧注射液對(duì)H1N1、H3N2、B流感病毒NA抑制活性較高，半數(shù)抑制濃度（IC50）分別為（46.49±2.25）、（49.77±1.77）、（45.33±5.32）μg/mL。金銀花聚酰胺柱洗脫濃縮液、醋酸乙酯萃取液及殘液、柱色譜提取物、隱綠原酸、異綠原酸A和異綠原酸B對(duì)H1N1、H3N2、B流感病毒NA酶活性均具有抑制作用。結(jié)論 熱毒寧注射液及其有機(jī)酸類成分對(duì)流感病毒神經(jīng)氨酸酶活性均具有一定抑制作用。 關(guān)鍵詞：熱毒寧注射液；流感病毒；神經(jīng)氨酸酶

Objective To research the inhibitory activity of Reduning Injection and its components on neuraminidases (NA) in influenza virus. Methods The inhibition of Reduning Injection and its constituents on NA in influenza virus was determined by measuring the fluorescence intensity. Results Reduning Injection had strong inhibition on NA in H1N1, H3N2, and B influenza virus, and their half inhibitory concentration (IC50) values were (46.49 ± 2.25), (49.77 ± 1.77), and (45.33 ± 5.32) μg/mL. Lonicerae Japonicae Flos extract as polyamide column wash off concentrate, ethyl acetate extract and residue, column chromatography extracts, implicit chlorogenic acid, different chlorogenic acid A, and different chlorogenic acid B had inhibitory activities on NA in H1N1, H3N2, and B influenza virus. Conclusion Reduning Injection and the organic acid from Lonicerae Japonicae Flos have certain inhibitory activities on NA in influenza viruses.

國(guó)家重大新藥創(chuàng)制項(xiàng)目（2013ZX09402203)