[關(guān)鍵詞]
[摘要]
目的 研究生半夏對(duì)HepG2細(xì)胞增殖和轉(zhuǎn)氨酶釋放量的影響。方法 分別用石油醚、醋酸乙酯、95%乙醇、純水提取生半夏,將提取物作用于HepG2細(xì)胞,設(shè)置對(duì)照組、5-氟尿嘧啶(100 mg/L)組和半夏提取物組(劑量分別為0.1、1、10、20 mg/L)。24 h后,MTT法測(cè)定生半夏對(duì)HepG2細(xì)胞增殖的影響;48 h后,測(cè)定培養(yǎng)上清中谷丙轉(zhuǎn)氨酶(ALT)、天冬氨酸轉(zhuǎn)氨酶(AST)的水平。結(jié)果 與對(duì)照組相比,生半夏各組分在劑量不高于10 mg/L時(shí)毒性很小,20 mg/L對(duì)細(xì)胞增殖有較強(qiáng)抑制作用,并顯著性提高ALT釋放量。不同組分的細(xì)胞毒性強(qiáng)弱趨勢(shì)為石油醚>醋酸乙酯>95%乙醇>純水。結(jié)論 生半夏對(duì)HepG2細(xì)胞有一定細(xì)胞毒性,表現(xiàn)為抑制細(xì)胞增殖和損傷細(xì)胞膜,其作用強(qiáng)度與劑量、提取溶劑有關(guān)。 關(guān)鍵詞:生半夏;HepG2細(xì)胞;細(xì)胞增殖;轉(zhuǎn)氨酶釋放
[Key word]
[Abstract]
Objective To study the effect of Pinelliae Rhizoma on the cell proliferation and transaminase release of HepG2 cells. Methods Ligroin, acetidin, 95% ethanol, and water were used to extract the components from Pinelliae Rhizoma, and the extracts were added to HepG2 cells. Six groups such as normal (without drugs), control (5-fluorouracil, 100 mg/L), and Pinelliae Rhizoma extracts (0.1, 1, 10, and 20 mg/L) groups were set up. After 24 h, cell multiplication was determined by MTT assay. After 48 h, glutamicpyruvic transaminase (ALT) and aminotransferase (AST) in supernatant of culture medium were tested by kits. Results Compared with the control group, the toxicity of Pinelliae Rhizoma was weak when the dosages were no higher than 10 mg/L. When the dosage came to 20 mg/L, the toxicity was strong, and the release of ALT was enhanced significantly. The precedence ordering of toxicity of different extracts was ligroin > acetidin> 95% ethanol > water. Conclusion There are toxicity of Pinelliae Rhizoma on HepG2, and it is reflected by the inhibition of cell proliferation and cell membrane injury. The intensity is related with the processing methods and extracting solvent.
[中圖分類號(hào)]
[基金項(xiàng)目]
國(guó)家重大新藥創(chuàng)制專項(xiàng)(2011ZX09102-011-08)