[關(guān)鍵詞]
[摘要]
目的 考察枸杞提取液對(duì)球形紅細(xì)菌生長(zhǎng)的影響。方法 在不同質(zhì)量濃度的常規(guī)培養(yǎng)基中添加不同質(zhì)量濃度的枸杞提取液,培養(yǎng)球形紅細(xì)菌,比較球形紅細(xì)菌的活菌數(shù)、生長(zhǎng)曲線和脫氫酶活性,考察枸杞提取液對(duì)球形紅細(xì)菌生長(zhǎng)的影響。結(jié)果 在培養(yǎng)基中添加枸杞提取液可縮短球形紅細(xì)菌生長(zhǎng)延遲期,使其提前進(jìn)入指數(shù)期和穩(wěn)定期;當(dāng)質(zhì)量濃度為12.5 g/L時(shí),球形紅細(xì)菌活菌數(shù)增加到之前的1.5倍,使球形紅細(xì)菌脫氫酶活性提高到之前的3.3倍。結(jié)論 培養(yǎng)基中添加一定量的枸杞提取液可以促進(jìn)球形紅細(xì)菌的生長(zhǎng),使球形紅細(xì)菌生長(zhǎng)周期縮短、活力提高。
[Key word]
[Abstract]
Objective To study the effects of Lycium barbarum extract on the growth of Rhodobacter sphaeroides. Methods Adding L. barbarum extract into general culture medium at different concentration, the effects of L. barbarum extract on the growth of R. sphaeroides were investigated by culturing R. sphaeroide and comparing living R. sphaeroides bacteria number, growth curve, and dehydrogenase activity.. Results After adding L. barbarum extract into the culture medium, the growth delay period of R. sphaeroides was shortened and the expotential and stationary periods were advanced. When the concentration of L. barbarum extract was 12.5 g/L, the number of living R. sphaeroides was increased in 1.5 times, and the dehydrogenase activity of R. sphaeroides was improved in 3.3 times as much as before. Conclusion When L. barbarum extract is added into the culture medium, the growth and the activity of R. sphaeroides could be improved, and the growth cycle could be shortened.
[中圖分類號(hào)]
[基金項(xiàng)目]
國(guó)家自然科學(xué)基金資助項(xiàng)目(81173473);山西省自然科學(xué)基金資助項(xiàng)目(2010011048-2);太原市科技項(xiàng)目人才專項(xiàng)明星專題項(xiàng)目(120247-08);山西醫(yī)科大學(xué)青年基金(02201128,02201124)