目的 建立茯苓皮中松苓新酸的HPLC測定方法。方法 采用高效液相色譜法，Lichrospher C18色譜柱（200 mm×4.6 mm，5 μm），流動相為乙腈–0.5%磷酸水溶液（80∶20），檢測波長為242 nm，體積流量為1.0 mL/min，柱溫為30 ℃，進樣量為10 μL。結(jié)果 松苓新酸在0.142 5～2.280 0 μg與峰面積呈良好的線性關(guān)系（r=0.999 3），平均回收率為101.83%，RSD值為1.45%（n=6）。結(jié)論 該方法操作簡便，穩(wěn)定可行，重復(fù)性好，可用于茯苓皮中松苓新酸的測定。

Objective To establish an HPLC method for the determination of dehydrotrametenolic acid in the peels of Poria cocos. Methods HPLC was carried out on a Lichrospher C18 column (200 mm×4.6 mm, 5 μm) with acetonitrile-0.5% phosphoric acid (80∶20) as mobile phase. The detection wavelength was set at 242 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The column temperature was at 30 ℃. Results The calibration curve was linear over the range of 0.142 5—2.280 0 μg (r=0.999 3) for dehydrotrametenolic acid. The average recovery rate was 101.83% with RSD value at 1.45% (n=6). Conclusion The established method is rapid, sensitive, and accurate, and could be used for the determination of dehydrotrametenolic acid in the peels of P. cocos.