R值分別為9.585、6.483 min。最佳工藝為:厚樸60%乙醇提取物,用10倍量的硅膠進行裝柱,以石油醚-醋酸乙酯(10:1~10:3)作為梯度洗脫溶劑,體積流量選擇5 mL/min。結(jié)論 與傳統(tǒng)工藝相比,采用閃式硅膠柱色譜法以厚樸總提物為原料,能夠快速、高效純化得到質(zhì)量分數(shù)>99.5%厚樸總酚。;Objective To optimize the separation and purification of total phenols from Magnolia officinalis with flash silica gel column chromatography. Methods The content and yield of total phenols were used as makers to optimize the purification of total phenols from the method of sample pretreatment, dosage of silica gel, elution solvent, and flow rate by flash silica gel column chromatography. Results Magnolol and honokiol had a good linearity in the range of 1-100 μg/mL (r =0.999 9). The tR values of magnolol and honokiol were 9.585 and 6.483 min, respectively. The optimal processing technology was using 60% ethanol extract with the amount of 10 times silica gel, and the mobile phase was petroleum ether-ethyl acetate (10:1-10:3) in gradient elution mode. The flow rate was controlled at 5 mL/min. Conclusion Compared with the traditional method, using flash silica gel chromatography with the total extract is easy to obtain the total magnolol with the purity more than 99.5% quickly."/>

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首頁 > 過刊瀏覽>2014年第29卷第7期 >2014,29(7):721-724. DOI:10.7501/j.issn.1674-5515.2014.07.005
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閃式硅膠柱色譜法分離純化高純度厚樸總酚的研究

Separation and purification of total phenols from Magnolia officinaliswith flash silica gel column chromatography

發(fā)布日期:2014-07-26