目的 建立馬齒莧藥材中總多酚的測定方法，考察不同產(chǎn)地馬齒莧藥材中總多酚，為馬齒莧藥材質量評價提供參考。方法 以沒食子酸為對照品，采用福林酚（FC）比色法測定馬齒莧中總多酚。結果 不同產(chǎn)地的馬齒莧藥材中多酚含量在6.30～17.37 mg/g。結論 FC比色法簡單快速，穩(wěn)定，結果準確，適用于馬齒莧中總多酚的測定；不同產(chǎn)地馬齒莧中總多酚差異較大，建議馬齒莧藥材中總多酚質量分數(shù)以沒食子酸計不低于6 mg/g。

Objective To establish a method for determination of total polyphenols in Portulaca oleracea, to investigate the total polyphenols in this herb from various habitats, and to provide reference for quality evaluation of P. oleracea. Methods The total polyphenols content in P. oleracea was determined by Folin-Ciocalteu (FC) colorimetric method with gallic acid as the standard. Results The total polyphenols of 11 batches of P. oleracea varied from 6.30 to 17.37 mg/g. Conclusion FC colorimetric method is a quick, easy-operating, precise, and stable method, which is suitable for the determination of total polyphenols in P. oleracea. In consideration of big differences of total polyphenols among P. oleracea from various habitats, it is suggested that the total polyphenols in P. oleracea crude drug should be above 6 mg/g.

國家自然科學基金面上項目（81073005）；山東大學自主創(chuàng)新項目（2012TS102）