目的 研究熱毒寧注射液體外抑制甲型H1N1流感病毒的作用。方法 以奧司他韋為陽性對(duì)照，采用CPE和MTT法觀察熱毒寧注射液對(duì)甲型H1N1流感病毒的抑制作用。結(jié)果 CPE法結(jié)果表明熱毒寧注射液最大無毒濃度（TC₀）為16.2 mg/mL，半數(shù)中毒濃度為（TC₅₀）為（24.5±8.1）mg/mL；MTT法測(cè)定結(jié)果表明熱毒寧注射液TC₀為16.2 mg/mL，TC₅₀為（21.7±9.4）mg/mL。熱毒寧注射液體外抑制甲型H1N1流感病毒結(jié)果顯示，CPE法和MTT法熱毒寧注射液作用感染細(xì)胞1次組半數(shù)有效濃度（IC₅₀）為（900.0±173.2）、（933.3±57.7）μg/mL，治療指數(shù)（TI）為27.2、23.2；熱毒寧注射液作用感染細(xì)胞3次組IC₅₀為（666.7±115.5）、（866.7±208.1）μg/mL，TI為36.7、25.0。結(jié)論 熱毒寧注射液具有明顯體外抗甲型H1N1流感病毒的作用。

Objective To study the inhibitory effects of Reduning Injection on influenza A/H1N1 influenza virus in vitro. Methods With Oseltamivir as positive control, CPE and MTT methods were used to observe inhibitory effects of Reduning Injection on influenza A/H1N1 influenza virus in vitro. Results The CPE method results showed that maximum of no toxicity concentration (TC₀) of Reduning Injection was 16.2 mg/mL, and median toxic concentration (TC₅₀) was (24.5 ± 8.1) mg/mL. The MTT method results showed that maximum of no toxicity concentration (TC₀) was (16.2 ± 0) mg/mL, and median toxic concentration (TC₅₀) was (21.7 ± 9.4) mg/mL. The in vitro inhibition of influenza A/H1N1 influenza virus of Reduning Injection showed the administration of Reduning Injection to infected cells once, median toxic concentration (TC₅₀) of the CPE method and the MTT method were (900.0 ± 173.2) μg/mL, (933.3 ± 57.7) μg/mL, therapeutic indexes (TI) were 27.2 and 23.2, and administration of Reduning Injection to infected cells for three times, median toxic concentration (TC₅₀) of the CPE method and the MTT method were (666.7 ± 115.5) μg/mL, (866.7 ± 208.1) μg/mL, TI were 36.7 and 25.0. Conclusion Reduning Injection has obvious resistance to the influenza A/H1N1 influenza virus in vitro. Administration of Reduning Injection to infected MDCK cells for three times is superior to the administration of Reduning Injection to infected MDCK cells once.

國家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃（973計(jì)劃）項(xiàng)目（2010CB735604）