目的 研究三七皂苷K對(duì)照品的制備。方法 利用大孔吸附樹(shù)脂從三七剪口提取液中分離得三七總皂苷，再用反相柱色譜法分離得到三七皂苷K，并根據(jù)理化性質(zhì)和波譜數(shù)據(jù)鑒定化合物的結(jié)構(gòu)，同時(shí)運(yùn)用薄層色譜（TLC）、高效液相色譜（HPLC）法檢查及測(cè)定質(zhì)量分?jǐn)?shù)。結(jié)果 三七皂苷K單體經(jīng)TLC檢查無(wú)雜質(zhì)斑點(diǎn)，二維及三維HPLC測(cè)定三七皂苷K的質(zhì)量分?jǐn)?shù)大于99%，峰質(zhì)量分?jǐn)?shù)因數(shù)為998.40。結(jié)果表明上述制得的純品為單一化合物。結(jié)論 制備的三七皂苷K達(dá)到中藥質(zhì)量標(biāo)準(zhǔn)用化學(xué)對(duì)照品的技術(shù)要求。

Objective To study the preparation of notoginsenoside K reference substance. Methods The total saponins in the roots and rhizomes of Panax pseudoginseng were isolated from the roots and rhizomes of P. pseudoginseng by macroporous adsorption resin, then notoginsenoside K was isolated by reverse phase column chromatography. Their structures were identified on the basis of physicochemical properties and spectroscopic data. TLC and HPLC were employed for inspecting its purity and content. Results The TLC experiments showed the notoginsenoside K had no impurity spot. 2D and 3D HPLC showed a single peak with a purity factor of 998.4 and the content of notoginsenoside K was above 99%. Conclusion The prepared notoginsenoside K complies with the technical requirements of quality standard in Chinese materia medica with chemical reference substance.

云南省科技廳重大科技專(zhuān)項(xiàng)（2012ZA002）