目的 探討腰痹通膠囊改善大鼠腰椎間盤突出癥以及其自身免疫調(diào)節(jié)的機(jī)制。方法 取大鼠自身尾椎髓核，移植入腰椎神經(jīng)根部位以建立腰椎間盤突出模型，并隨機(jī)分為模型組、腰痛寧膠囊（0.16 g/kg）組和腰痹通膠囊低、中、高劑量（0.17、0.34、0.68 g/kg）組以及對(duì)照組、假手術(shù)組。給藥30 d后，檢測(cè)大鼠左后肢壓痛痛閾、血清免疫球蛋白IgG和IgM水平、外周血T淋巴細(xì)胞亞群CD4⁺和CD8⁺比例及神經(jīng)根部位病理變化。結(jié)果 與模型組比較，腰痹通膠囊高、中劑量組大鼠痛閾顯著升高（P<0.01、0.05），同時(shí)腰痹通膠囊高劑量組大鼠痛閾增加值明顯降低（P<0.01）；腰痹通膠囊高、中劑量組能顯著降低大鼠血清IgG水平（P<0.01），同時(shí)腰痹通膠囊高劑量組能顯著降低大鼠血清IgM水平（P<0.05）；腰痹通膠囊高、中劑量組能顯著降低大鼠CD4⁺含量（P<0.01、0.05），同時(shí)腰痹通膠囊高劑量組能顯著降低大鼠CD4⁺/CD8⁺值（P<0.05）。組織病理圖顯示腰痹通膠囊組局部軸索、神經(jīng)纖維病變均有不同程度的改善，提示腰痹通膠囊能改善大鼠腰椎間盤突出模型神經(jīng)根病變。結(jié)論 腰痹通膠囊可以改善腰椎間盤突出大鼠模型的癥狀和神經(jīng)根病變，其機(jī)制可能與調(diào)節(jié)機(jī)體自身免疫狀態(tài)有關(guān)。

Objective To study the improvement of Yaobitong Capsules on prolapse of lumbar intervertebral disc in rats and discuss its mechanism about autoimmune regulator. Methods The model of prolapse of lumbar intervertebral disc was established by transplant of autologous nucleus pulposus of rats. All the rats were randomly divided into model group, Yaotongning Capsules (0.16 g/kg) group, low-, mid-, and high-dose of Yaobitong Capsule (0.17, 0.34, and 0.68 g/kg) groups, control group, and Sham group. After the administration for 30 d, the mechanical pain thresholds of left hind limb, IgG, IgM levels in serum, the ratio between T-lymphocyte subsets CD4⁺ and CD8⁺ cells in the peripheral blood, and pathological changes in the nerve root of rats were detected, respectively. Results Compared with the model group, the mechanical pain thresholds of rats in the mid-, and high-dose of Yaobitong Capsule groups were increased significantly (P<0.01, 0.05), and added value of pain threshold of rats in the high-dose of Yaobitong Capsule groups was obviously reduced (P<0.01). The IgG levels in serum of rats in the mid-, and high-dose of Yaobitong Capsule groups were decreased obviously (P<0.01), and the IgM levels in the high-dose of Yaobitong Capsule groups were also decreased significantly (P<0.01). The contents of CD4⁺ in the mid- and high-dose of Yaobitong Capsule groups were obviously reduced (P<0.01, 0.05), and CD4⁺/CD8⁺ value in the high-dose of Yaobitong Capsule groups was also reduced significantly (P<0.05). The pathological changes indicated that local axonal and nerve fibers of rats in Yaobitong Capsule groups were to some extent improved, and obviously improved in the nerve root. Conclusion Yaobitong Capsules can reduce the sensitivity of mechanical pain, improve the symptoms and nerve root lesions of rats in the model of prolapse of lumbar intervertebral disc, which can be related with regulating autoimmune abnormalities caused by herniated disc in the body.

國(guó)家重大新藥創(chuàng)制項(xiàng)目（2013ZX09402203）