目的 建立大孔樹脂富集純化蜘蛛香總黃酮工藝方法。方法 對(duì)D-101、AB-8、HPD-600、D-301 4種不同極性大孔樹脂進(jìn)行吸附率和解吸率篩選，確定選擇HPD-600樹脂?？疾炝薍PD-600樹脂上樣量、pH值、上樣體積流量、上樣液濃度、洗脫液配比、酸液濃度、乙醇體積分?jǐn)?shù)、洗脫劑用量對(duì)蜘蛛香總黃酮的影響，優(yōu)化大孔樹脂純化工藝參數(shù)。結(jié)果 HPD-600樹脂裝柱，上樣濃度為3.03 mg/mL，上樣容量為24.24 mg/g，先0.1 mol/L HCl的10%乙醇溶液洗脫除雜，直至洗液顏色不再變淺、固形物含量不再增加，后用0.1 mol/L HCl的80%乙醇溶液，洗脫體積流量1 mL/min，洗脫5倍柱體積，收集洗脫液減壓濃縮至干得提取物。結(jié)論 HPD-600樹脂適用于蜘蛛香中總黃酮的純化，可使蜘蛛香總黃酮質(zhì)量分?jǐn)?shù)達(dá)55.25%，收率可達(dá)39.01%。

Objective To establish a method of purification process for total flavonoids from Valeriana jatamansi. Methods To select an optimum resin according to the adsorption rate and resolution rate from D-101, AB-8, HPD-600, and D-301, and study the factors such as adsorption amount, pH value, elution speed, concentration of adsorption, solution proportion, concentration of hydrochloric acid, concentration of ethanol, and amount of eluent, which could affect the adsorption and desorption behavior. Results The HPD 600 resin among four types of resins was found with the best separating efficiency. The best results of dynamic absorption were based on the followings: sample concentration was 3.03 mg/mL, feed rate 1 mL/min, and sample capacity 24.24 mg/g. After eluted with 0.1 mol/L HCl of 10% ethanol and 5 BV of 0.1 mol/L HCl of 80% ethanol. Conclusion HPD-600 resin could be used to purify the total flavonoids of V. jatamansi, and he yield of total flavones was 39.01% and the purity was 55.25%.

湖北省自然科學(xué)基金重點(diǎn)項(xiàng)目（2010CDA047）