反相高效液相色譜（RP-HPLC）中的流動(dòng)相對(duì)組分具有很強(qiáng)的親和力，并且與固定相共同競(jìng)爭(zhēng)組分，流動(dòng)相的種類及配比直接影響到組分的分離。因此，正確的選擇流動(dòng)相，確定最佳的色譜分離條件，可以實(shí)現(xiàn)更理想的分離。結(jié)合日常實(shí)驗(yàn)經(jīng)驗(yàn)和相關(guān)文獻(xiàn)，綜述了在RP-HPLC中，如何快速地進(jìn)行流動(dòng)相的選擇與優(yōu)化。

The mobile phase of reversed phase high performance liquid chromatography (RP-HPLC) has a strong affinity for the components and competes for the components with the stationary phase. The species and proportion of the mobile phase directly affect the separation of components. Therefore, correctly choosing the mobile phase and determining the best chromatographic conditions could achieve better separation. Combining daily experiences with related literatures, the selection and optimization of mobile phase in RP-HPLC has been discussed.