目的 探討聚乙二醇化重組人生長激素(PEG-rhGH)對去垂體幼鼠主要臟器的影響.方法 SD雄性幼鼠隨機取88只進行去垂體手術造模,另18只假手術組為對照組.術后2周,選成模幼鼠54只,分為模型組、重組人生長激素(rhGH)組、PEG-rhGH組.每晚sc生理鹽水0.25 mg/kg,并且rhGH組每晚sc注射用重組人生長激素0.25 mg/kg,PEG-rhGH組每周第1天晚上sc注射用重組人生長激素1.40 mg/kg.治療4周后,檢測肝功能ALT、AST、TBIL、DBIL水平,腎功能BUN、CREA水平,心肌酶譜CK、CKMB及肝臟、心臟、腎臟的病理組織和肝臟的轉錄生長因子β1(TGF-β1)和腎臟C-fos蛋白表達.結果 肝功能中AST、TBIL水平下降;腎功能中CREA水平升高;肝臟、腎臟、心臟病理未見明顯異常;肝臟TGF-β1、腎臟C-fos蛋白表達組間無差異.結論 PEG-rhGH對肝臟、腎臟、心臟的組織形態(tài)和功能無明顯不良影響.

Objective To investigate the effects of polyethylene glycol recombinant human growth hormone (PEG-rhGH) on the major organs of hypophysectomized rats. Methods Eighty-eight Sprague-Dawley young male rats were underwent hypophysectomy, and another 18 rats with Sham operations were selected into the control group. Fifty-four qualified rats were randomly divided into the model, rhGH, and PEG-rhGH groups after two weeks. Saline was used with sc administration at dosage of 0.25 mg/kg every night. Recombinant Human Growth Hormone for Injection (0.25 mg/kg) was sc given every night in rhGH group, but Polyethylene Glycol Recombinant Human Growth Hormone for Injection (1.40 mg/kg) was sc given in first night every week in PEG-rhGH group. After treatment for 4 weeks, Liver function such as ALT, AST, TBIL, and DBIL levels, renal function such as BUN and CREA levels, myocardial enzymes CK, CKMB, and liver, heart, kidney pathology transcription, and the protein expression of transforming growth factor-β1 (TGF-β1) and renal C-fos in liver tissue were determined. Results There were decrease of levels of AST and TBIL in liver function, but increase of CREA level in renal function. No obvious abnormalities of pathology in liver, kidney, and heart. There were no difference for hepatic TGF-β1 and the kidneys C-fos protein expression among groups. Conclusion No significant adverse effects of PEG-rhGH are observed on the liver, kidney, and heart morphology and function.