目的 建立超高效液相色譜(UPLC)同時測定熱炎寧顆粒中大黃素、咖啡酸、綠原酸、野黃芩苷、木犀草素、虎杖苷6種成分的方法.方法 采用UPLC法,Acquity UPLC HSS C₁₈色譜柱(100 mm×2.1 mm,1.7 μm);流動相為[甲醇-乙腈(35:65)]-0.1%磷酸水溶液為流動相,梯度洗脫,體積流量0.5 mL/min,分段變波長測定,柱溫35 ℃;進(jìn)樣量1.0 μL.結(jié)果 6種成分在選定的范圍內(nèi)線性關(guān)系良好(r≥0.999 3);平均加樣回收率在99.08%～102.31%,RSD值均小于2.53%(n=6).結(jié)論 采用UPLC法對熱炎寧顆粒中大黃素、咖啡酸、綠原酸、野黃芩苷、木犀草素、虎杖苷6種成分進(jìn)行測定,方法操作快速,高效,準(zhǔn)確,可用于熱炎寧顆粒的質(zhì)量控制.

Objective To develop a UPLC method for simultaneous determination of emodin, caffeic acid, chlorogenic acid, scutellarin, luteolin, and polydatin in Reyanning Granules. Methods UPLC chromatography was used. The chromatographic separation was carried out on an Acquity UPLC HSS C₁₈ column (100 mm × 2.1 mm, 1.7 μm) with [methanol - acetonitrile (21:79)] - 0.1% phosphoric acid as mobile phases at the flow rate of 0.5 mL/min for gradient elution. Detection with variable wavelength was used, and the column temperature was 35 ℃ with injection volume of 1.0 μL. Results There were good separations for six components in the specific ranges (r≥0.999 3). The average recovery was in the range of 99.08% — 102.31% with RSD value less than 2.53% (n = 6). Conclusion Simultaneous determination method of emodin, caffeic acid, chlorogenic acid, scutellarin, luteolin, and polydatin in Reyanning Granules by UPLC is established, and is accurate, sensitive, credible, and repeatable which can be applied to the quality control for Reyanning Granules.

天津市中醫(yī)藥管理局中醫(yī)中西醫(yī)結(jié)合科研項(xiàng)目(13140)