目的 建立穿山龍藥材的超高效液相色譜(UPLC)特征指紋圖譜分析方法。方法 采用Agilent Poroshell 120 Bonus-RP色譜柱(50 mm×3.0 mm, 2.7 μm);流動相:乙腈-水, 梯度洗脫;體積流量:0.8 mL/min;檢測波長:203 nm;柱溫:25 ℃;進樣量:10 μL。應(yīng)用相似度分析軟件建立穿山龍藥材指紋圖譜的共有模式, 并對色譜峰進行指認(rèn)。結(jié)果 建立了穿山龍藥材的UPLC特征指紋圖譜共有模式, 標(biāo)定了14個共有峰, 指認(rèn)了其中3個共有峰, 10批穿山龍藥材的相似度為0.9以上。通過聚類分析, 將13批穿山龍藥材聚為4類。結(jié)論 該方法快速, 可用于評價穿山龍藥材質(zhì)量。

Objective To establish a UPLC method for determination the characteristic chromatographic profiles of Dioscoreae Nipponicae Rhizoma. Methods The UPLC characteristic chromatographic profiles was carried on Agilent Poroshell 120 Bonus-RP column (50 mm × 3.0 mm, 2.7 μm) with acetonitrile-water as mobile phase in gradient elution. The detective wavelength was set at 203 nm. The temperature of column was set at 25 ℃. The flow rate was 0.8 mL/min with injection volume of 10 μL. The UPLC characteristic chromatographic profiles of Dioscoreae Nipponicae Rhizoma were established with similarity analyses and the peaks were identified. Results The common mode of the UPLC characteristic chromatographic profile was set up. There were 14 common peaks, and three of them were identified. The similarities of 10 samples to the common mode were above 0.9. The 13 batches of samples were classified into four clusters by cluster analysis. Conclusion The method is rapid, and can be used for the quality control for Dioscoreae Nipponicae Rhizoma.

天津市科技計劃項目(12ZCDZSY11600、14ZCZDSY00005);天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計劃項目(14JCQNJC13800)