目的 研究白芍總苷體外對(duì)不同化學(xué)體系和生物體系的抗氧化活性。方法 通過(guò)Fenton反應(yīng)生成·OH自由基,采用DPPH·自由基,觀察不同質(zhì)量濃度的白芍總苷溶液對(duì)化學(xué)體系自由基的清除作用。利用鄰二氮菲-Fe²⁺-H₂O₂產(chǎn)生的·OH自由基造成紅細(xì)胞膜破裂,以及肝、腦細(xì)胞脂質(zhì)的過(guò)氧化,觀察不同質(zhì)量濃度的白芍總苷溶液對(duì)生物體系自由基的清除作用。結(jié)果 白芍總苷對(duì)·OH有一定的清除作用,其IC₅₀為0.62 g/L。白芍總苷對(duì)DPPH·自由基有較強(qiáng)的清除作用,IC₅₀為6.6 mg/L;對(duì)·OH自由基引發(fā)的紅細(xì)胞膜破裂有一定的抑制作用,其IC₅₀為30.17 mg/L,對(duì)肝、腦勻漿脂質(zhì)過(guò)氧化有一定的抑制作用,其IC₅₀分別為6.9、16.3 mg/L。結(jié)論 白芍總苷具有清除自由基作用,在不同體外實(shí)驗(yàn)體系中抗氧化量效關(guān)系存在差異。

Objective To study antioxidant activities in vitro of total glucosides of paeony (TGP) on various chemical system and biological system. Methods Hydroxyl radical (·OH) was generated by Fenton reaction and DPPH· radicals were used. Scavenging effects of various concentrations of TGP on free radicals from different chemical antioxidant systems were evaluated. Red cell membrane rupture and the liver or brain homogenate lipid peroxidation caused by OH from phenanthridine-Fe²⁺ -H₂O₂ system were used to evaluate scavenging effects of various concentrations of TGP on free radicals from different chemical biological systems. Results TGP had a certain effect to scavenge ·OH free radical with the IC₅₀ value of 0.62 g/L. TGP also had stronger effect to scavenge DPPH· free radical with the IC₅₀ value of 6.6 mg/L, and had inhibition of red cell membrane rupture with the IC₅₀ value of 30.17 mg/L, and lipid peroxidation with the IC₅₀ value of 6.9, 16.3 mg/L, respectively. Conclusion TGP has antioxidant abilities, and there were differences of antioxidant activity potency among differ experiment systems.