目的 建立同時測定接骨丸中芍藥苷、柚皮苷、橙皮苷、丹酚酸B、丹參酮Ⅱ_A的HPLC-DAD方法。方法 采用高效液相色譜波長切換法。Megres C₁₈色譜柱(250 mm×4.6 mm,5 μm);以乙腈-0.1%磷酸溶液為流動相,進(jìn)行梯度洗脫;檢測波長切換為0～15 min為230 nm、15～45 min為280 nm、45～68 min為270 nm;體積流量1.0 mL/min;柱溫30 ℃;進(jìn)樣體積10 μL。結(jié)果 芍藥苷在35.27～352.70 ng、柚皮苷在8.625～86.250 μg、橙皮苷在53.11～531.10 μg、丹酚酸B在19.34～193.40 μg、丹參酮Ⅱ_A在2.142～21.420 μg時進(jìn)樣量與峰面積積分值的線性關(guān)系良好。芍藥苷、柚皮苷、橙皮苷、丹酚酸B、丹參酮Ⅱ_A的平均回收率分別為100.47%、96.78%、97.45%、100.25%、99.94%,RSD值分別為0.48%、1.07%、1.11%、1.18%、1. 83%。結(jié)論 本法簡便、快速、準(zhǔn)確,可同時測定接骨丸中芍藥苷、柚皮苷、橙皮苷、丹酚酸B、丹參酮Ⅱ_A。

Objective To establish a quantification method for simultaneous determination of paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone Ⅱ_A in Jiegu Pills by HPLC-DAD. Methods HPLC simultaneously with changing ultraviolet-visible wavelength was used. Megres C₁₈ column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-0.1% phosphoric acid solution as mobile phase in gradient elution mode. The detection wavelengths were set at 230 nm (retention time 0-15 min), 280 nm (retention time 15-45 min), and 270 nm (retention time 45-68 min), respectively. Injection volume was 10 μL at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃. Results There were good linear relationships between peak areas and contents of five components, such as paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone Ⅱ_A in the ranges of 35.27-352.70 ng, 8.625-86.250 ng, 53.11-531.10 ng, 19.34-193.40 ng, and 2.142-21.420 ng. The average recoveries of five components were 100.54%, 96.78%, 97.45%, 100.25%, and 99.94% with RSD values of 0.48%, 1.07%, 1.11%, 1.18%, and 1.83%, respectively. Conclusion The established method is convenient, accurate with a satisfactory separation, and high sensitivity. It can be used to determine paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone Ⅱ_A in Jiegu Pills with the same chromatogram condition.