目的 建立測定腦血栓片中苦杏仁苷、羥基紅花黃色素A、芍藥苷、阿魏酸、丹酚酸B和丹參酮Ⅱ_A的HPLC-DAD法。方法 采用HPLC-DAD法,Agilent Poroshell 120 SB-C₁₈色譜柱(100 mm×4.6 mm,2.7 μm);流動相:甲醇-0.2%磷酸溶液,梯度洗脫;檢測波長分別為210 nm(苦杏仁苷)、403 nm(羥基紅花黃色素A)、230 nm(芍藥苷)、321 nm(阿魏酸)、286 nm(丹酚酸B)、270 nm(丹參酮Ⅱ_A);體積流量:0.7 mL/min;柱溫:30 ℃;進(jìn)樣量3～5 μL。結(jié)果 苦杏仁苷、羥基紅花黃色素A、芍藥苷、阿魏酸、丹酚酸B和丹參酮Ⅱ_A 6個成分的線性范圍分別為11.90～1 158.90、9.14～91.39、11.70～1 173.50、4.04～1 011.00、3.97～992.20、4.40～551.00 ng;平均回收率分別為96.47%、96.92%、99.96%、97.20%、97.57%、96.50%,RSD值分別為1.3%、1.6%、1.3%、1.7%、1.9%、0.7%。結(jié)論 所建立的方法可同時測定腦血栓片中苦杏仁苷、羥基紅花黃色素A、芍藥苷、阿魏酸、丹酚酸B和丹參酮Ⅱ_A。

Objective To develop an HPLC-DAD method for determination of amygdalin, hydroxysafflor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, and tanshinoneⅡ_A in Naoxueshuan Tablets. Methods HPLC-DAD chromatography was used. The analysis was carried out on an Agilent Poroshell 120 SB-C₁₈ column (100 mm × 4.6 mm, 2.7 μm) with methanol - 0.2% phosphoric acid as mobile phases at the flow rate of 0.7 mL/min for gradient elution. Detection with variable wavelength were used, and set at 210 nm for amygdalin, 403 nm for hydroxysafflor yellow A, 230 nm for paeoniflorin, 321 nm for ferulic acid, 278 nm for salvianolic acid, and 270 nm for tanshinoneⅡ_A. The column temperature was 30 ℃ with injection volume of 3 —5 μL. Results The linear ranges of six components were 11.90 — 1158.90, 9.14 — 91.39, 11.70 — 1 173.50, 4.04 — 1 011.00, 3.97 — 992.20, 4.40 — 551.00 ng, respectively. The average recoveries were 96.47%, 96.92%, 99.96%, 97.20%, 97.57%, and 96.50% with RSD1.3%, 1.6%, 1.3%, 1.7%, 1.9%, and 0.7%, respectively. Conclusion This method can be used to simultaneously determine amygdalin, hydroxysafflor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, and tanshinoneⅡ_A in Naoxueshuan Tablets.