目的 研究新疆阿魏的提取物、不同極性部位以及倍半萜香豆素類化合物對(duì)人肺癌細(xì)胞的體外抑制作用,明確新疆阿魏樹(shù)脂的活性成分.方法 應(yīng)用MTT法檢測(cè)新疆阿魏樹(shù)脂提取物(100 mg/mL)、不同極性部位(100 mg/mL)和倍半萜香豆素化合物(100、50、10、1、0.1 μmol/L)對(duì)人肺癌細(xì)胞系A(chǔ)549、H292、H1792、H460作用48 h后生長(zhǎng)的抑制作用,采用抑制率和IC₅₀為指標(biāo)衡量其活性.結(jié)果 新疆阿魏樹(shù)脂氯仿提取物在100 mg/mL質(zhì)量濃度下對(duì)A549、H292、H1792、H460細(xì)胞系的抑制率分別為75.45%、65.39%、46.54%、73.68.低極性部位在100 mg/mL質(zhì)量濃度下對(duì)4種細(xì)胞系的生長(zhǎng)抑制率分別為88.74%、80.62%、60.11%、92.35%.從低極性部位中分離得到的主要倍半萜香豆素類化合物farnesiferol B、farnesiferone A、farnesiferol C、多花素寧,其中farnesiferol C、多花素寧的IC₅₀分別為35.49、15.44、44.25、21.63 μmol/L和23.60、17.90、38.33、12.35 μmol/L,表現(xiàn)出顯著的生長(zhǎng)抑制作用,且具有劑量相關(guān)性.結(jié)論 新疆阿魏樹(shù)脂的低極性部位為體外抑制人肺癌細(xì)胞生長(zhǎng)作用的活性部位,其所含有的高含量倍半萜香豆素化合物farnesiferol C為其主要活性成分.

Objective To study inhibitory action in vitro on human lung cancer cells of extract, different fractions, and sesquiterpene coumarins from Ferula sinkiangensis resin, and to clarify its effective constituent. Methods Using MTT method, inhibitory actions of chloroform extract (at concentration of 100 mg/mL), different polarity fractions (at concentration of 100 mg/mL), and sesquiterpene coumarins (at different concentrations of 100, 50, 10, 1, and 0.1 μmol/L) from F. sinkiangensis resin were tested on A549, H292, H1792, and H460 cell lines after 48 h co-incubation. The inhibition rate and IC₅₀ values were calculated to assay their effects. Results The chloroform extract from F. sinkiangensis resin exhibited significant inhibitory action at 100 mg/mL against A549, H292, H1792, and H460 human lung cancer cells with inhibition rates as 75.45%, 65.39%, 46.54%, and 73.68%, respectively. And the low polarity fraction had inhibitory action at 100 mg/mL against the four human lung cancer cells with inhibition rates as 88.74%, 80.62%, 60.11%, and 92.35%, respectively. Sesquiterpene coumarins farnesiferol B, farnesiferone A, farnesiferol C, and polyanthinin were isolated from low polarity fraction, and farnesiferol C and polyanthinin showed significant inhibitory action on growth of human lung cancer cells with dose dependent manner. The IC₅₀ values of farnesiferol C were 35.49, 15.44, 44.25, and 21.63 Conclusion Low polarity fraction is considered as the effective part of F. sinkiangensis resin for inhibitory activities against human lung cancer cell lines, and the sesquiterpene coumarin farnesiferol C isolated from it is main active constituent.

沈陽(yáng)藥科大學(xué)大學(xué)生創(chuàng)新創(chuàng)業(yè)計(jì)劃項(xiàng)目