目的 觀察羥苯磺酸鈣對糖尿病大鼠視網(wǎng)膜病變的影響并探討其機制。方法 以65 mg/kg一次性ip鏈脲佐菌素誘導糖尿病模型,將糖尿病模型大鼠分為模型組、復方血栓通組(1.05 g/kg)和羥苯磺酸鈣組(0.334 g/kg),另設對照組,均ig給藥,1次/d,對照組給予等劑量蒸餾水,共12周。采用Real-time PCR法檢測Ⅳ型膠原及血管內皮生長因子(VEGF)mRNA在視網(wǎng)膜組織中的表達;光鏡下觀察視網(wǎng)膜血管形態(tài)學改變,測定視網(wǎng)膜毛細血管面積密度;透視電鏡觀察大鼠視網(wǎng)膜超微結構。結果 與對照組比較,模型組大鼠視網(wǎng)膜Ⅳ型膠原mRNA表達升高(P < 0.05),VEGF mRNA升高(P < 0.01),毛細血管面積密度顯著增高(P < 0.001)。與模型組比較,羥苯磺酸鈣組大鼠視網(wǎng)膜Ⅳ型膠原mRNA、VEGF mRNA表達和毛細血管面積密度均降低(P < 0.05)。結論 羥苯磺酸鈣能下調視網(wǎng)膜中Ⅳ型膠原、VEGF的表達,抑制纖維組織增生和血管新生,從而改善糖尿病視網(wǎng)膜病變。

Objective To observe the effect of calcium dobesilate on the diabetic retinopathy in rats and to explore its mechanism. Methods Rats were ip administered with streptozotocin (65 mg/kg) to induce diabetes model. The diabetic rats were randomly divided into model group, Compound Xueshuantong group (1.05 g/kg), and calcium dobesilate group (0.334 g/kg). Other rats were recruited as the control group. The expression of collagen Ⅳ mRNA and vascular endothelial growth factor (VEGF) mRNA in retinas of rats were determined by Real-time PCR method. The morphology was observed under light microscope, and retinal capillary areas were determined. The ultrastructure of retinal capillary was observed under electron microscope. Results Compared with the control group, the indexes of rats in model group such as collagen Ⅳ mRNA expression (P < 0.05), VEGF mRNA expression (P < 0.01), and capillary density (P < 0.001) significantly increased. Compared with the model group, collagen Ⅳ mRNA, VEGF mRNA expression, and capillary density in calcium dobesilate group were significantly decreased (P <0.05). Conclusion Calcium dobesilate can down-regulate the expressions of collagen Ⅳ and VEGF in retinas of rats, restrain fibrous tissue hyperplasia and angiogenesis, thereby improve the diabetic retinopathy.

國家科技支撐計劃項目(2012BAI29B07);北京中醫(yī)藥大學在讀研究生項目(2014-JYBZZ-XS-004)