目的 建立同時測定八味腎氣丸中去氫土莫酸、茯苓酸、梓醇、桃葉珊瑚苷4個成分的高效液相色譜測定方法。方法 采用Hypersil C₁₈色譜柱(250 mm×4.6 mm,5 μm);流動相為乙腈-0.1%磷酸溶液,梯度洗脫;檢測波長：0～18 min,241 nm(去氫土莫酸);18～50 min,210 nm(茯苓酸、梓醇和桃葉珊瑚苷);體積流量為1.0 mL/min;柱溫25 ℃;進(jìn)樣量20 μL。結(jié)果 去氫土莫酸、茯苓酸、梓醇和桃葉珊瑚苷分別在3.35～67.00 μg/mL、3.50～70.00 μg/mL、3.07～61.40 μg/mL、3.25～65.00 μg/mL線性良好,r值均大于0.999 0,平均回收率分別為96.72%、97.87%、98.21%、98.09%,RSD值分別為1.08%、0.95%、0.64%、1.22%(n=6)。結(jié)論 該方法簡便,結(jié)果準(zhǔn)確,可用于八味腎氣丸中去氫土莫酸、茯苓酸、梓醇、桃葉珊瑚苷的質(zhì)量控制。

Objective To establish an HPLC method for simultaneous determination of dehydrotumulosic acid, pachymic acid, catalpol, and aucubin in Bawei Shenqi Pills. Methods The analysis was performed on Hypersil C₁₈ column (250 mm × 4.6 mm, 5 μm) with acetonitrile - 0.1% phosphoric acid solution as mobile phases for gradient elution. Detection with variable wavelength was used, and set at 241 nm for dehydrotumulosic acid, 210 nm for pachymic acid, catalpol, and aucubin. The flow rate was 1.0 mL/min. The column temperature was 25 ℃ with injection volume of 20 μL. Results The calibration curve was linear over the concentration range of 3.35 — 67.00 μg/mL for dehydrotumulosic acid, 3.50 — 70.00 μg/mL for pachymic acid, 3.07 — 61.40 μg/mL for catalpol, and 3.25 — 65.00 μg/mL for aucubin, respectively. The correlation coefficients of all curves were above 0.999 0. The average recoveries were 96.72%, 97.87%, 98.21%, and 98.09% with RSD 1.08%, 0.95%, 0.64%, and 1.22% (n=6), respectively. Conclusion The method is simple and accurate, which can be used in quantity control of dehydrotumulosic acid, pachymic acid, catalpol, and aucubin in Bawei Shenqi Pills.