目的 研究桑葉多糖對小鼠腸道菌群失調(diào)的調(diào)節(jié)作用。方法 利用鹽酸林可霉素誘導(dǎo)小鼠腸道菌群失調(diào)。將造模成功的小鼠隨機(jī)分為桑葉多糖組、乳酶生組、模型組, 另設(shè)置對照組。桑葉多糖組小鼠ig 6.0 mg/mL桑葉多糖溶液0.6 mL(劑量0.144 mg/g), 乳酶生組小鼠ig 5.5 mg/mL乳酶生混懸液0.6 mL(劑量0.132 mg/g)。2次/d, 連續(xù)給藥7 d。模型組、對照組小鼠自行飲水。取小鼠的新鮮糞便, 提取腸道菌DNA。16S段DNA序列擴(kuò)增, 變性梯度凝膠電泳(DGGE)法分析。比對各條帶及樣品, 進(jìn)行聚類分析, 對各組小鼠腸道微生物群落結(jié)構(gòu)進(jìn)行主成分分析。結(jié)果 DGGE直觀圖分析, 經(jīng)過自然恢復(fù)、桑葉多糖和乳酶生的作用, 小鼠腸道菌群種類有了明顯的增加, 但與對照組小鼠的腸道菌群比較仍然有差別, 并不能完全恢復(fù)到原來的水平。聚類分析結(jié)果可以看出, 與對照組最接近的是乳酶生組, 其次是桑葉多糖組, 最后是自然恢復(fù)組。菌群落結(jié)構(gòu)的主成分分析可以直觀地看出, 桑葉多糖、乳酶生組小鼠腸道菌群與對照組關(guān)系最近, 模型組與對照組腸道菌群及其他組腸道菌群的差異明顯。結(jié)論 桑葉多糖對小鼠腸道菌群失調(diào)有調(diào)節(jié)作用。

Objective To study the regulation of Morus alba polysaccharides on disturbance of intestinal flora in mice. Methods Models of disturbance of intestinal flora in mice were induced by lincomycin hydrochloride. Mice with disturbance of intestinal flora were randomly divided into M. alba polysaccharides group, lactasin group, and model group. And other rats were selected as the control group. The mice in the M. alba polysaccharides group were ig administered with 6.0 mg/mL M. alba polysaccharides 0.6 mL (equivalent to dosage 0.144 mg/g), and those in lactasin group were ig administered with 5.5 mg/mL Lactasin Tablets 0.6 mL (equivalent to dosage 0.132 mg/g). The animals were given drugs twice daily, and lasted for 7 d. Mice in model and control groups were fed with water at the same time. DNA of intestinal flora was extracted from the fresh excrement of the mice. 16S DNA sequence amplification was done, and the analysis was determined via denaturing gradient gel electrophoresis (DGGE) method. Strips of samples were observed, the clustering analysis was carried out, and principal component analysis (PCA) method was used to analyze structures of intestinal flora in mice. Results It could be observed from DGGE that the species of intestinal flora in mice obviously increased after natural recovery, or treated by M. alba polysaccharides and lactasin. But there was still difference of the number of intestinal flora in mice between these three groups and the control group. The intestinal flora could not completely resume to the original level. The cluster analysis of these samples showed that control group was close to lactasin group, secondly M. alba polysaccharides, and then natural recovery group. Flora community structure PCA results of control group were near to those of M. alba polysaccharides and lactasin groups, however there were significant differences between model group and control group and other group. Conclusion M. alba polysaccharides has regulatory effects on disturbance of intestinal flora in mice.