目的 明確麩炒蒼術(shù)健脾的活性部位.方法 選用番瀉葉水煎液ig 制備大鼠脾虛模型.考察麩炒蒼術(shù)揮發(fā)油、二氯甲烷萃取物、正丁醇萃取物和多糖對(duì)脾虛大鼠體質(zhì)量、血清胃泌素、血管活性腸肽、血清淀粉酶和D-木糖水平的影響.結(jié)果 與模型組比較,除多糖組外其余各給藥組在治療后體質(zhì)量均明顯增加(P< 0.05);大鼠血清淀粉酶、D-木糖、血清胃泌素水平顯著增加,血管活性腸肽水平降低,差異均具有顯著性(P< 0.05).結(jié)論 除多糖無(wú)健脾作用外,麩炒蒼術(shù)其余各部位健脾作用明顯,并且健脾的活性部位主要為低極性成分.

Objective To confirm active parts from Atractylodis Rhizoma stir-baked with bran on invigorating the spleen. Methods Spleen insufficiency model was made by ig administration with extract of Sennae Folium. Body weight and levels of gastrin, vasoactive intestinal peptide, serum amylase, and D-xylose in the serum of rats were studied after ig administration with volatile oil, CH₂Cl₂ active parts, n-BuOH active parts, and polysaccharde. Results Compared with model group, the body weights in the volatile oil group, CH₂Cl₂ active parts, and n-BuOH active parts obviously increased (P < 0.05). And the levels of gastrin, serum amylase, and D-xylose were increased, but the levels of vasoactive intestinal peptide significantly decreased (P < 0.05). Conclusion Active parts except polysaccharide from Atractylodis Rhizoma stir-baked with bran show the functions of vigorating the spleen, and low polarity components are active.

國(guó)家自然科學(xué)基金資助項(xiàng)目(81202919);國(guó)家中醫(yī)藥管理局中醫(yī)藥行業(yè)科研專項(xiàng)項(xiàng)目(20110700712)