目的 探討金黃凝膠對大鼠急性軟組織損傷的治療作用.方法 隨機(jī)選取10 只大鼠作為對照組,將其余大鼠隨機(jī)分為模型組、雙氯芬酸鈉凝膠(0.010 g)組、青鵬軟膏(0.300 g)組、金黃散膏(0.069 g)組以及金黃凝膠0.033、0.069、0.138 g 組.采用砝碼砸傷法造成大鼠急性軟組織損傷.造模后24 h 開始給藥,各組分別以各受試藥物均勻涂抹大鼠左后肢砸傷部位.觀察砸傷部位腫脹程度,計算腫脹度;測定造模部位痛閾值;記錄損傷指數(shù);測定血清白細(xì)胞介素-1β(IL-1β)、白細(xì)胞介素-6(IL-6)、前列腺素E₂(PGE₂)、一氧化氮(NO)的含量;觀察造模部位的病理變化.結(jié)果 與模型組比較,給藥第3、5 天金黃凝膠0.033、0.069、0.138 g 組腫脹度均顯著降低、痛閾值顯著升高(P< 0.05、0.01);金黃凝膠0.033、0.069、0.138 g 組給藥5 d 后損傷指數(shù)顯著降低(P< 0.05、0.01).與模型組比較,金黃凝膠0.033、0.069、0.138 g 組PGE₂顯著降低(P< 0.01),金黃凝膠0.138 g 組IL-6、0.069 g 組NO 顯著降低(P< 0.05).各給藥組大鼠對病變均有不同程度改善,除金黃凝膠0.033 g 組外,各給藥組大鼠肌肉病變均顯著降低(P< 0.05、0.01).結(jié)論 金黃凝膠對急性軟組織損傷具有治療作用.

Objective To explore the therapeutic effects of Jinghuang Gel on acute soft tissue injury in rats. Methods Except 10 rats were selected as control group, the other rats were randomly divided into model group, Diclofenac Sodium Gel (0.010 g) group, Qingpeng Ointment (0.300 g) group, and Jinghuang Gel (0.033, 0.069, and 0.138 g) groups. Acute soft tissue injury models in rats were caused by crushing method. After 24 h, rats were smeared with different medicines on left hind limb injured site. The degree of swelling in injured site was observed, and swelling degree was calculated. Pain threshold was determined. Damage index was recorded. Levels of IL-1β, IL-6, PGE₂, and NO in serum were determined. And pathological changes were detected in injured site before and after the administration. Results Compared with the model group, swelling degrees of Jinghuang Gel (0.033, 0.069, and 0.138 g) groups were significantly decreased, and pain thresholds were obviously increased (P < 0.05, 0.01) after administration for 3 and 5 d; The damage index of those groups was significantly decreased (P < 0.05, 0.01) after administration for 5 d. Compared with the model group, levels of PGE₂ of Jinghuang Gel (0.033, 0.069, and 0.138 g) groups were significantly decreased (P < 0.05, 0.01), and levels of IL-6 in Jinghuang Gel 0.138 and 0.069 g groups were also significantly decreased (P < 0.05). The injury degree of rats administered was improved, and muscle injury scores were significantly decreased (P < 0.05, 0.01) except Jinghuang Gel 0.033 g group. Conclusion Jinghuang Gel has therapeutic effect on acute soft tissue injury.

國家重大新藥創(chuàng)制項目(2013ZX09402203)