目的 研究蘆丁對(duì)流感病毒的體外抑制作用并探討其機(jī)制。方法 利用流感病毒感染MDCK細(xì)胞模型,觀察蘆丁對(duì)流感病毒的體外抑制作用;結(jié)合流感病毒感染前、感染同時(shí)及感染后加藥的不同方式探索蘆丁作用于流感病毒生命周期的具體階段;采用熒光底物法考察蘆丁對(duì)流感病毒神經(jīng)氨酸酶的抑制作用。結(jié)果 蘆丁對(duì)A/PuertoRico/8/1934(H1N1)、A/FM1/1/47(H1N1)、A/Human/Hubei/3/2005(H3N2)、A/Beijing/32/92 (H3N2)4株流感病毒均具有體外抑制作用,其中對(duì)A/PuertoRico/8/1934(H1N1)株的半數(shù)有效濃度(EC₅₀)最小,選擇指數(shù)(SI)最大,體外藥效最好。蘆丁在流感病毒感染后給藥的效果最為明顯,對(duì)流感病毒A/FM1/1/47(H1N1)的IC₅₀最小,對(duì)神經(jīng)氨酸酶活性抑制作用相對(duì)最好。結(jié)論 蘆丁抑制了流感病毒神經(jīng)氨酸酶活性,具有較好的體外抗流感病毒作用。

Objective To study the inhibition of rutin against influenza virus in vitro and explore its mechanism. Methods To establish the MDCK cell models infected by influenza virus, and inhibition of rutin against influenza virus in vitro was observed. The specific stages of influenza virus infection treated by rutin were evaluated before, during and after infection. Inhibition of rutin against neuraminidase activities of influenza virus were studied by fluorescent substrate method. Results Rutin exhibited antiviral activity against A/PuertoRico/8/1934(H1N1), A/FM1/1/47(H1N1), A/Human/Hubei/3/2005(H3N2), and A/Beijing/32/92(H3N2), especially on A/PuertoRico/8/1934(H1N1) with minimum EC₅₀, maximum SI, and best efficacy in vitro. The effect is the most obvious treated by rutin administration after infected with influenza virus. With IC₅₀ value on A/FM1/1/47(H1N1) influenza virus minimum, rutin inhibited the relatively the best activity of neuraminidase. Conclusion Rutin inhibits activity of neuraminidase of influenza virus, and has good anti-influenza effects in vitro.

國(guó)家科技部重大新藥創(chuàng)制項(xiàng)目(2013ZX09402203)