目的 建立不同來源的千金藤植物中千金藤素的HPLC測定方法。方法 采用AngelaC₁₈色譜柱(250mm×4.6mm,5μm);流動相:乙腈–水(52:48,含0.5%三乙胺、0.01%磷酸);檢測波長:282nm;柱溫:30℃;體積流量:1.0mL/min;進樣體積:20μL。結(jié)果 千金藤素質(zhì)量濃度在9~216μg/mL時與峰面積值呈良好的線性關(guān)系(r＝0.9997),平均回收率為96.0%,RSD值為2.0%(n＝6)。產(chǎn)自湖南的千金藤(No.13)、產(chǎn)自廣西的白藥子(No.17)中千金藤素的量較高,分別為0.0139%、0.1221%;同一植株的不同用藥部位(No.12~15)中千金藤素的量也不盡相同,湖南產(chǎn)千金藤中千金藤素主要分布在莖中,其質(zhì)量分數(shù)為0.0139%。結(jié)論 該方法操作簡便、準確、重復性好,可用于千金藤植物千金藤、地不容和白藥子中千金藤素的測定。

Objective To establish an HPLC method for determination of cepharanthine in the plants of StephaniaLour. from various habitats and parts. Methods The determination was carried out on Angela C₁₈ column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water (52:48, 0.5% triethylamine and 0.01% phosphoric acid). The column temperature was set at 30 ℃ at a flow rate of 1.0 mL/min. The injection volume was 20 μL. Results Cepharanthine had a good linearity in the ranges of 9-216 μg/mL (r = 0.999 7). The average recovery rate was 96.0% with RSD value at 2.0%. Contents of cepharanthine in Stephania japonica sample (No.13) from Hunan province and S.cepharantha sample (No.17) from Guangxi province were the highest. Contents of cepharanthine in various plant parts of samples (No.12-15) were different, and that in S. japonica from Hunan province were generally found in the stem, which content reached to 0.013 9%. Conclusion The established method is simple, sensitive, and highly reproducible, which is suitable for the determination of cepharantine in S. japonica, S. epigaea, and S.cepharantha.

國家自然科學基金資助項目(U1403102,81473108);遼寧省自然科學基金資助項目(2015020732)