目的 觀察消巖湯對環(huán)磷酰胺誘導(dǎo)Lewis肺癌小鼠造血微環(huán)境的影響,探究消巖湯對Lewis肺癌小鼠骨髓抑制的造血微環(huán)境的改善作用及其作用機(jī)制。方法 SPF級Lewis肺癌小鼠隨機(jī)分為對照組、模型組、重組人粒細(xì)胞刺激因子組和消巖湯組,每組各10只。對照組和模型組ig 0.02 mL/g生理鹽水。人粒細(xì)胞刺激因子組在造模后sc重組人粒細(xì)胞刺激因子注射液15 ng/g,1次/d,連續(xù)給藥7 d。消巖湯組在造模后ig 2.9 g/mL消巖湯,2次/d,0.2 mL/次,連續(xù)給藥7 d。采用流式細(xì)胞儀分析細(xì)胞周期,通過細(xì)胞培養(yǎng)技術(shù)檢測基質(zhì)細(xì)胞分化能力,采用QRT-PCR法檢測骨髓細(xì)胞Bcl-2和Bax mRNA的基因表達(dá)。結(jié)果 與模型組比較,消巖湯能促進(jìn)骨髓細(xì)胞從G₀/G₁期向S、G₂/M期轉(zhuǎn)化(P<0.05);能一定程度上緩解化療導(dǎo)致的成纖維細(xì)胞集落生成單位(CFU-F)形成減少(P<0.05);能上調(diào)抗凋亡基因Bcl-2 mRNA的表達(dá)((P<0.05)。結(jié)論 消巖湯能夠促進(jìn)骨髓細(xì)胞向增殖周期轉(zhuǎn)化,上調(diào)Bcl-2 mRNA表達(dá),改善造血微環(huán)境,從而緩解Lewis肺癌骨髓抑制對小鼠造血功能的影響。

Objective To observe the effects of Xiaoyan Decoction on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression induced by cyclophosphamide, and discuss the improvement on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression and to explore its mechanism. Methods SPF Lewis lung cancer mice were randomly divided into control, model, recombinant human granulocyte colony-stimulating factor (rhG-CSF), and Xiaoyan Decoction groups, and each group had 10 mice. Mice in the control and model groups were ig normal saline 0.02 mL/g. Mice in the rhG-CSF group were sc Recombinant Human Granulocyte Colony-Stimulating Factor Injection 15 ng/g after be established myelosuppression models, once daily, and be treated for 7 d. Mice in the Xiaoyan Decoction group were ig Xiaoyan Decoction 2.9 g/mL afterbe established myelosuppression models, twice daily, 0.2 mL/time, and be treated for 7 d. The cell cycles were analyzed by flow cytometry, differentiation of stromal cell were determined by cell culture technique, and express levels on Bcl-2 mRNA and Bax mRNA of bone marrow cells were checked by qRT-PCR method. Results Compared with the model group, Xiaoyan decoction could promote the bone marrow cells from G₀/G₁ phase to S and G₂ /M phase transformation in the cell cycle (P < 0.05), could inhibit the reduction of fibroblast colony forming unit (CFU-F) formation induced by chemotherapy to a certain extent(P < 0.05), and could up-regulate the expression of anti apoptotic gene Bcl-2 mRNA (P < 0.05). Conclusion Xiaoyan Decoction can promote the transformation of bone marrow cells to proliferation cycles, up-regulate the expression of Bcl-2 mRNA gene, improve hemopoietic microenvironment, and relieve the effect of hematopoietic function in Lewis lung cancer mice with myelosuppression.

國家自然科學(xué)基金資助項目(81273937);天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計劃項目(13JCQNJC10900);天津市科技計劃項目(12ZCDZSY15800)