目的 建立HPLC法同時測定青貫解毒顆粒中去甲氧基莢果蕨素、重樓皂苷Ⅶ、重樓皂苷Ⅱ和重樓皂苷Ⅰ。方法 采用Agilent Zorbax SB C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相A：甲醇-乙腈（2∶1），流動相B：水，采用梯度洗脫；0～32 min時在298 nm波長下檢測去甲氧基莢果蕨素，32～75 min在203 nm波長下檢測重樓皂苷Ⅶ、重樓皂苷Ⅱ和重樓皂苷Ⅰ；體積流量：0.9 mL/min；進樣量：20 μL。結(jié)果 去甲氧基莢果蕨素、重樓皂苷Ⅶ、重樓皂苷Ⅱ和重樓皂苷Ⅰ分別在4.14～82.80 μg/mL（r=0.9999）、3.80～76.00 μg/mL（r=0.9995）、4.94～98.80 μg/mL（r=0.9998）、7.57～151.40 μg/mL（r=0.9997）與峰面積具有較好的線性關(guān)系。平均回收率分別為99.26%、97.62%、98.27%、98.90%，RSD值分別為0.87%、1.39%、1.14%、1.15%。結(jié)論 建立的HPLC法同時測定青貫解毒顆粒中去甲氧基莢果蕨素、重樓皂苷Ⅶ、重樓皂苷Ⅱ和重樓皂苷Ⅰ，方法操作準確、簡便，可作為青貫解毒顆粒的質(zhì)量控制方法。

Objective To develop an HPLC method for determination of demethoxymatteucinol, chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰ in Qingguan Jiedu Granules. Methods The determination was carried out on Agilent Zorbax SB C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of phase of A:methanol-acetonitrile (2:1) and phase B:water with gradient elution. The detection wavelengths were 298 nm in 0-32 min (determination of demethoxymatteucinol) and 203 nm in 32-75 min (determination of chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰ). The flow rate was 0.9 mL/min, and volume of injection was 20 μL. Results There were good linear relationships of demethoxymatteucinol, chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰ in the concentration ranges of 4.14-82.80 μg/mL (r=0.9999), 3.80-76.00 μg/mL (r=0.9995), 4.94-98.80 μg/mL (r=0.9998), and 7.57-151.40 μg/mL (r=0.9997) between peak areas, respectively. The average recoveries were 99.26%, 97.62%, 98.27%, and 98.90% with RSD 0.87%, 1.39%, 1.14%, and 1.15%, respectively. Conclusion The established method can been successfully used for simultaneous determination of demethoxymatteucinol, chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰin Qingguan Jiedu Granules, and the method is accurate and simple which can be used in quantity control for Qingguan Jiedu Granules.