目的 研究紫雪散對大鼠膠原誘導(dǎo)性關(guān)節(jié)炎的治療作用及其機制研究。方法 Wistar大鼠隨機分為5組，分別為對照組、模型組、吲哚美辛片（2 mg/kg）組以及紫雪散0.5、1.0 g/kg組，每組10只。建立大鼠Ⅱ型膠原誘導(dǎo)性關(guān)節(jié)炎模型。大鼠初次免疫后第12天開始灌胃給藥，給藥容量10 mL/kg，1次/d。對照組、模型組均灌胃給予同體積生理鹽水。各組均持續(xù)給藥3周。測量大鼠左后足踝寬度、厚度和周長，計算關(guān)節(jié)炎指數(shù)。觀察大鼠踝關(guān)節(jié)病理。采用ELISA法測定血清炎癥因子血小板活化因子（PAF）水平、環(huán)氧合酶-2（COX-2）活性、腫瘤壞死因子α（TNF-α）水平，采用比濁法測定血清循環(huán)免疫復(fù)合物（CIC）水平。結(jié)果 給藥7 d，紫雪散組踝關(guān)節(jié)寬度、厚度顯著低于模型組并能持續(xù)到給藥21 d。紫雪散組滑膜細胞增生明顯減輕，滑膜組織充血水腫明顯減輕。與模型組比較，紫雪散組全身表現(xiàn)評分、關(guān)節(jié)腫脹指數(shù)和關(guān)節(jié)炎指數(shù)均顯著降低（P<0.05、0.01、0.001）。與模型組比較，紫雪散0.5、1.0 g/kg組血清PAF水平顯著減少，COX-2活性顯著降低（P<0.001）。與模型組比較，紫雪散0.5、1.0 g/kg組血清TNF-α、CIC水平顯著減少（P<0.05、0.01、0.001）。結(jié)論 紫雪散對大鼠膠原誘導(dǎo)性關(guān)節(jié)炎損傷具有治療效果，其機制可能與紫雪散的抗炎和抗氧化作用有關(guān)。

Objective To investigate the therapeutic effects of Zixue Powder on collagen-induced arthritis in rats and its mechanism. Methods Wistar rats were randomly divided into control group, model group, Indometacin Tablets (2 mg/kg) group, and Zixue Powder (0.5 and 1.0 g/kg) groups, and each group had 10 mice. Type Ⅱ collagen-induced arthritis model in rats was established. Rats were ig administered from 12th day after the first immunization, and the volume was 10 mL/kg, 1 time/d. Rats in the control group and model group were given the same volume of normal saline. All animals were treated for 3 weeks. Left ankle joint width, thickness, and perimeter were determined, and arthritis indexes were calculated. Pathology of rat ankle joint was observed. The lever of PAF, COX-2, and TNF-α were determined by ELISA method, and level of circulating immune complex was determined by turbidimetry method. Results Treatment for 7 d continue to 21 d, ankle joint width and thickness of rats in the Zixue Powder groups were lower than those of rats in model group. The proliferations of synovial cells were significantly reduced in the Zixue Powder groups, and synovial tissue edema was also significantly reduced. Compared with model group, systemic manifestations score, joint swelling index, and arthritis index in the Zixue Powder group were significantly decreased (P<0.05, 0.01, and 0.001). Compared with model group, levels of serum PAF in the Zixue Powder groups were significantly reduced, activities of COX-2 were significantly decreased (P<0.01), and levels of CIC and TNF-α were significantly decreased (P<0.05, 0.01, and 0.001).Conclusions Zixue Powder has therapeutic effects on collagen-induced arthritis in rats, which may be in regard to the antiinflammatory properties and anti-oxidant activity.