目的 建立HPLC法同時測定參茸安神片中遠(yuǎn)志酮Ⅲ、3，6'-二芥子?；崽?、細(xì)葉遠(yuǎn)志皂苷、遠(yuǎn)志皂苷B和五味子醇甲的方法。方法 采用Kromasil C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相：乙腈-0.1%磷酸溶液，梯度洗脫；檢測波長：320 nm（0~16 min檢測遠(yuǎn)志酮Ⅲ、3，6'-二芥子?；崽牵?10 nm（16~24 min檢測細(xì)葉遠(yuǎn)志皂苷、遠(yuǎn)志皂苷B）、250 nm（24~35 min檢測五味子醇甲）；體積流量：1.0 mL/min；柱溫：30℃；進(jìn)樣量為10 μL。結(jié)果 遠(yuǎn)志（口山）酮Ⅲ、3，6'-二芥子?；崽恰⒓?xì)葉遠(yuǎn)志皂苷、遠(yuǎn)志皂苷B和五味子醇甲分別在3.85~77.00、6.22~124.40、6.57~131.40、7.61~152.20、4.96~99.20 μg/mL線性關(guān)系良好（r≥0.999 2）；平均回收率分別為98.17%、99.24%、99.90%、97.63%、96.83%，RSD值分別為1.14%、1.23%、0.70%、1.52%、0.81%。結(jié)論 本法穩(wěn)定可靠、簡便易行，為全面控制參茸安神片的質(zhì)量提供了參考。

Objective To establish an HPLC method for simultaneous determination of polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in Shenrong Anshen Tablets. Methods The determination was carried out with Kromasil C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution. The detection wavelengths were 320 nm in 0-16 min (determination of polygalaxanthone Ⅲ and 3,6'-disinapoyl sucrose), 210 nm in 16-24 min (determination of tenuifolin and onjisaponin B), and 250 nm in 24-35 min (determination of schisandrin). The flow rate was 1.0 mL/min, and the column temperature was set at 30℃ with injection volume of 10 μL. Results There were good linear relationships of polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in the concentration ranges of 3.85-77.00, 6.22-124.40, 6.57-131.40, 7.61-152.20, 4.96-99.20 μg/mL(r≥0.999 2). The average recoveries were 98.17%, 99.24%, 99.90%, 97.63%, and 96.83% with RSD 1.14%, 1.23%, 0.70%, 1.52%, and 0.81%, respectively. Conclusion The method is simple and valid, which can be used in quantity control for Shenrong Anshen Tablets.