[關鍵詞]
[摘要]
目的 評價澤瀉湯95%乙醇、50%乙醇和水提取物抑制HepG2肝細胞脂肪累積能力。方法 采用油酸誘導HepG2細胞脂肪累積建立模型,以噻唑蘭染色和油紅O染色法吸光度值為指標,結合細胞內脂滴形態(tài),評價澤瀉湯對油酸誘導HepG2細胞脂肪累積的干預作用。結果 澤瀉湯提取物作用HepG2細胞后,經染色后均發(fā)現(xiàn)細胞內脂滴明顯減少,且質量濃度越高脂類含量越少,50%乙醇提取物和水提物在終質量濃度160、320 μg/mL時,細胞內脂滴與模型組相比明顯降低(P<0.01),95%乙醇提物80 μg/mL作用細胞24 h后,細胞內脂滴較模型組有減少(P<0.05)。結論 3種澤瀉湯提取物均能夠減少細胞內脂肪的堆積,其中以50%乙醇提取物對HepG2細胞脂肪堆積抑制能力最強。
[Key word]
[Abstract]
Objective To evaluate the effects of 95% ethanol, 50% ethanol, and distilled water extracts from Zexie Decoction on fat accumulation of HepG2 hepatic cells. Methods Fat accumulation model in HepG2 cells was induced by oleic acid. Absorptions of MTT method and oil red O method and intracellular lipid droplet morphology were used to evaluate the effect of Zexie Decoction on oleic acid-induced fat accumulation in HepG2 cells. Results The lipid droplet of HepG2 cells was significantly reduced after cultivation with Zexie Decoction extracts, and the higher concentration, the less lipid contents. After culturing with 50% ethanol extract (160 μg/mL) and water extract (320 μg/mL), the lipid droplet of HepG2 cells reduced significantly compared with model group (P<0.01), and after culturing with 95% ethanol extract with the maximum concentration of 80 μg/mL for 24 h. the lipid droplet of HepG2 cells was also reduced (P<0.05). Conclusion Three extracts of Zexie Decoction show the effect of lipid-decreasing, and 50% ethanol extract is the best lipid-lowering ingredient.
[中圖分類號]
[基金項目]
天津市應用基礎與前沿技術研究計劃項目(14JCQNJC13800;天津市科技計劃項目(14ZCZDSY00005)