目的 建立毛細(xì)管電泳法測(cè)定（S）-2-（6-羥基-2,3-二氫苯并呋喃-3-基）乙酸甲酯中R異構(gòu)體的方法。方法 采用毛細(xì)管電泳法。以磺酸-β-環(huán)糊精（S-β-CD）為選擇劑；25 mmol/L硼酸鹽緩沖液（pH 8.9，含S-β-CD 1.8%）為運(yùn)行緩沖液；運(yùn)行電壓為25 kV；柱溫為15℃；檢測(cè)波長(zhǎng)：214 nm；3.4 kPa壓力進(jìn)樣10 s。結(jié)果 （S）-2-（6-羥基-2,3-二氫苯并呋喃-3-基）乙酸甲酯與R異構(gòu)體的分離度為2.9。R異構(gòu)體在2~30 μg/mL與峰面積線(xiàn)性關(guān)系良好（r=0.999 5），平均回收率為96.6%，RSD值為4.9%（n=6）。結(jié)論 建立的毛細(xì)管電泳法操作簡(jiǎn)便，結(jié)果準(zhǔn)確可靠，可用于（S）-2-（6-羥基-2,3-二氫苯并呋喃-3-基）乙酸甲酯中R異構(gòu)體的控制。

Objective To establish a capillary electrophoresis method for the determination of R-optical isomer in (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate. Methods Capillary electrophoresis method was adopted. Sulfate-β-cyclodextrin (S-β-CD) was used as selector. The background buffer was 25 mmol/L borate buffer solution (pH 8.9, containing 1.8% S-β-CD). The separation was performed at 15 ℃ with the voltage of 25 kV and the wavelength was 214 nm. Pressure was 3.4 kPa and injected for 10 s. Results (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate and its R-enantiomer were basely resolved with resolution of 2.9. The calibration curve of R-enantiomer was linear in the concentration range of 2 - 30 μg/mL (r = 0.999 5). The average recovery was 96.6% with RSD 4.9% (n = 6). Conclusion The method is convenient, rapid, accurate, and can be used for the determination of (R)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate in (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran- 3-yl) acetate.

山東省自然科學(xué)基金杰出青年基金資助項(xiàng)目（JQ201319）；山東大學(xué)自主創(chuàng)新基金資助項(xiàng)目（2016JC018）