目的 采用多種實驗模型評價注射用復方骨肽的藥效學，并探討其作用機制。方法 采用實驗性大鼠脛骨骨折模型觀察注射用復方骨肽的治療作用；采用小鼠扭體及福爾馬林痛模型觀察注射用復方骨肽的鎮(zhèn)痛作用；采用大鼠足腫和小鼠耳腫模型觀察注射用復方骨肽的抗炎作用。結(jié)果 與模型組比較，給藥2周，注射用復方骨肽15、45 mg/kg組血清轉(zhuǎn)化生長因子β1（TGF-β1）、骨形成蛋白（BMPs）水平明顯升高；給藥3周骨折程度明顯減輕，愈合率明顯升高（P< 0.05、0.01）。與對照組比較，注射用復方骨肽30、90 mg/kg組明顯減少扭體次數(shù)（P< 0.05、0.01）；注射用復方骨肽90 mg/kg明顯減少舔足時間。與模型組比較，注射用復方骨肽15、45 mg/kg組明顯減輕角叉菜膠引起的大鼠足腫脹。與對照組比較，注射用復方骨肽90 mg/kg明顯減輕二甲苯引起的耳腫脹。結(jié)論 注射用復方骨肽具有明顯的鎮(zhèn)痛、消炎作用，對實驗性骨折有明顯的治療作用，作用機制可能是促進生長因子TGF-β1、BMPs的合成，加快骨折愈合。

Objective To evaluate the pharmacodynamics of Compound Ossotide for injection by using various models, and to explore its mechanism. Methods Therapeutic effect of Compound Ossotide for injection was observed by using fracture model of rats tibia induced by hacksaw. Analgesic effect of Compound Ossotide for injection was observed by using mouse twist and faure marin model. Anti-inflammation effect of Compound Ossotide for injection was observed by using the model of rats ankle swelling caused by carrageenin and mice auris swelling caused by dimethylbenzene. Results Compared with model control group treated for two weeks, serum contents of TGF-β1 and BMPs in Compound Ossotide for injection 15 and 45 mg/kg groups were significantly increased. And treated for three weeks, the healing degree of fracture and healing rate were obviously improved (P < 0.05, 0.01). Compared with control group, the number of twisting caused by acetic acid in Compound Ossotide for injection 30 and 90 mg/kg groups were significantly reduced (P < 0.05, 0.01), and the licking foot time after injecting methanol in mice Compound Ossotide for injection 90 mg/kg group was significantly decreased. Compared with model group, Compound Ossotide for injection (90 mg/kg) could obviously reduce the foot swelling induced by carrageenin in rats. Compared with control group, Compound Ossotide for injection (15 and 45 mg/kg) could obviously reduce ear swelling induced by xylene. Conclusion Compound Ossotide for injection have obvious effects of analgesia and anti-inflammatory. The mechanism may be to promote the synthesis of growth factors TGF-β1 and BMPs and accelerate the healing of fracture.