目的 探討槲皮素對(duì)人胃癌MKN45細(xì)胞凋亡的影響及其作用機(jī)制。方法 取對(duì)數(shù)生長期的MKN45細(xì)胞分為對(duì)照組和20、40、80 μmol/L槲皮素組。應(yīng)用MTT比色法測定細(xì)胞活性，Hoechst染色、流式細(xì)胞技術(shù)檢測細(xì)胞凋亡，比色法檢測凋亡相關(guān)因子Caspase-3、Caspase-9表達(dá)。結(jié)果 槲皮素能顯著抑制MKN45細(xì)胞增殖，促進(jìn)細(xì)胞凋亡，升高Caspase-3、Caspase-9基因表達(dá)水平。結(jié)論 槲皮素可通過增強(qiáng)Caspase-3、Caspase-9基因表達(dá)誘導(dǎo)人胃癌MKN45細(xì)胞凋亡。

Objective To investigate the effect and mechanism of quercetin on MKN45 cells. Methods MKN45 cells were divided into four group: negative control group and quercetin groups (20, 40, and 80 μmol/L). Cell viability was measured by MTT colorimetric assay, apoptosis was detected by Hoechst staining and flow cytometry assay, and expression of apoptosis-related factors Caspase-3 and Caspase-9 were tested by colorimetric detection. Results Quercetin could obviously inhibit MKN45 cell proliferation and promote apoptosis, and could increase Caspase-3 and Caspase-9 gene expression levels. Conclusion Quercetin can induce gastric carcinoma MKN45 cells apoptosis by enhancing the expression of Caspase-3 and Caspase-9 gene.

天津市中醫(yī)中西醫(yī)結(jié)合科研課題（2015054）