目的 研究苦參堿對(duì)神經(jīng)膠質(zhì)瘤U87細(xì)胞增殖和侵襲的影響，探討其作用機(jī)制。方法 采用MTT法和Transwell侵襲實(shí)驗(yàn)分別檢測(cè)苦參堿對(duì)U87細(xì)胞的增殖能力和侵襲能力。采用Western blotting法和qPCR法檢測(cè)苦參堿對(duì)U87細(xì)胞的COX2蛋白和mRNA表達(dá)情況。結(jié)果 藥物作用24 h時(shí)，與對(duì)照組比較，隨著苦參堿濃度（5、50、100 μg/mL）的增加，苦參堿對(duì)U87細(xì)胞生長(zhǎng)的抑制率不斷地增加；相同濃度下，苦參堿對(duì)U87細(xì)胞增殖率在24、48、72 h時(shí)逐漸出現(xiàn)顯著性差異（P< 0.05）。對(duì)照組通過(guò)的細(xì)胞數(shù)量明顯多于苦參堿100 μg/mL組，兩組比較差異具有統(tǒng)計(jì)學(xué)意義（P< 0.01）。與對(duì)照組比較，隨著苦參堿濃度的增加，COX-2的蛋白和mRNA表達(dá)水平明顯降低，差異有統(tǒng)計(jì)學(xué)意義（P< 0.05、0.01）。結(jié)論 苦參堿通過(guò)降低COX-2的蛋白和mRNA表達(dá)來(lái)抑制U87細(xì)胞的增殖和侵襲。

Objective To investigate the influence of matrine on glioma U87 proliferation and invasion, and explore its mechanisms. Methods MTT and Transwell were used to detect the influence of matrine on multiplication capacity and invasion ability of U87 cells. Western blotting and qPCR methods were used to detect the COX2 protein and mRNA expression. Results Compared with the control group, inhibition rates of matrine treated for 24 h on the growth rate of U87 cells were increased with the concentrations of matrine (5, 50, 100 g/mL) increased. Under the same concentration, there were significant difference of inhibition rates of matrine treated for 24, 48, and 72 h on the proliferation of U87 cells (P < 0.05). The number of cells in the control group was significantly more than that in matrine 100 g/mL group, with significant differences between two groups (P < 0.01). Compared with the control group, the expression of COX-2 protein and mRNA in matrine groups were significantly decreased with the increase of matrine concentration, and the difference was statistically significant (P < 0.05, 0.01). Conclusion Matrine can inhibit the proliferation and invasion of U87 cells by lowering expression of COX-2 protein and mRNA.