目的 建立同時測定復(fù)方雙花片中綠原酸、咖啡酸、連翹苷、穿心蓮內(nèi)酯和木犀草素的方法。方法 采用Agilent 5 TC-C₁₈色譜柱（150 mm×4.6 mm，5 μm），流動相：乙腈–0.4%磷酸溶液，梯度洗脫；檢測波長切換0～16 min（327 nm）、16～30 min（228 nm）；體積流量：1.0 mL/min；柱溫：30 ℃，進(jìn)樣量：10 μL。結(jié)果 綠原酸、咖啡酸、連翹苷、穿心蓮內(nèi)酯和木犀草素在161.6～1616.0 ng（r=1.000 0）、12.48～124.80 ng（r=0.999 9）、14.30～143.04 ng（r=0.999 8）、13.48～134.80 ng（r=1.000 0）、8.72～87.20 ng（r=1.000 0）線性關(guān)系良好；平均回收率分別為101.15%、98.72%、101.42%、99.15%、98.76%，RSD值分別為1.83%、2.58%、1.69%、1.20%、1.96%。結(jié)論 該方法操作簡單，專屬性強，為更好地評價復(fù)方雙花片質(zhì)量提供了參考。

Objective To develop a method for determination of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin in Compound Shuanghua Tablets by HPLC.Methods The separation was performed on Agilent 5TC-C₁₈ column (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile -0.4% phosphoric acid with gradient elution. The detection wavelengths were set at 327 nm in 0-16 min and 228 nm in 16-30 min. The flow rate was 1.0 mL/min, temperature of column was set at 30 ℃, and volume of injection was 10 μL. Results The linear range of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin were 161.6-1616.0 ng (r=1.000 0), 12.48-124.80 ng (r=0.999 9), 14.30-143.04 ng (r=0.999 8), 13.48-134.80 ng (r=1.000 0), 8.72-87.20 ng (r=1.000 0), respectively. The average recoveries were 101.15%, 98.72%, 101.42%, 99.15%, and 98.76% with RSD 1.83%, 2.58%, 1.69%, 1.20%, and 1.96%, respectively. Conclusion The method is simple and specific, and provides a reference for quality of Compound Shuanghua Tablets.