目的 研究不同采收期龍膽中龍膽苦苷、獐牙菜苦苷、獐牙菜苷的變化規(guī)律。方法 采用Welchrom C₁₈色譜柱（250mm×4.6 mm，5 μm）；流動(dòng)相：甲醇-水（25：75）；檢測(cè)波長(zhǎng)：270（龍膽苦苷）、240 nm（獐芽菜苦苷和獐牙菜苷）；體積流量：1.0 mL/min；柱溫：25℃；進(jìn)樣量為10 μL。分別采收9、10月上、中、下旬的龍膽，考察其中龍膽苦苷、獐牙菜苦苷、獐牙菜苷的變化。結(jié)果 在9-10月，龍膽中龍膽苦苷、獐牙菜苦苷和獐牙菜苷的量基本呈現(xiàn)同步上升、同步下降趨勢(shì)。在9月中旬最高，9月下旬最低。與9月下旬比較，10月上旬的量有所回升，而10月下旬的量再次呈下降趨勢(shì)。結(jié)論 龍膽藥材最佳采收季節(jié)為9月中旬，此時(shí)龍膽苦苷、獐芽菜苦苷和獐牙菜苷的量最高。

Objective To study the dynamics trends of gentiopicroside, swertiamarin, and sweroside from Gentianae Radix et Rhizoma in different harvesting time. Methods The determination was carried out on Welchrom C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-water (25:75). The detection wavelengths were 240 nm (determination of swertiamarin and sweroside) and 270 nm (determination of gentiopicroside). The flow rate was 1.0 mL/min, and the column temperature was set at 25℃ with injection volume of 10 μL. Gentianae Radix et Rhizoma were collected in upper-, middle-, and late September and October, and the contents of gentiopicroside, swertiamarin, and sweroside were compared. Results The content of gentiopicroside, swertiamarin, and sweroside in Gentianae Radix et Rhizoma were simultaneously rising or falling from September to October, and the maximum degree was in mid-September, but the minimum degree was in late September, Compared with those in September, the contents were gradually rising in early October, while gradually decreased in mid-October. Conclusion The optimal harvest time of Gentianae Radix et Rhizoma is mid-September when the content of gentiopicroside, swertiamarin, and sweroside are the highest.

國(guó)家中藥標(biāo)準(zhǔn)化項(xiàng)目（ZYBZH-Y-TJ-43）