目的 評價丹參飲對冠脈結(jié)扎大鼠心肌缺血的保護作用，并探討其作用機制。方法 雄性Wistar大鼠按體質(zhì)量隨機分為對照組、模型組、硝苯地平組、復方丹參片組和丹參飲低、中、高劑量組，每組各10只。硝苯地平劑量為0.167 mg/kg、復方丹參片劑量為10 g/kg，按10 mL/kg ig給藥；丹參飲按0.5、1.0、2.0 mL/kg（相當于原藥材0.27、0.54、1.08 g/kg）ig給藥。各組動物均連續(xù)給藥7 d。于第7天給藥30 min后通過結(jié)扎冠脈建立大鼠心肌缺血模型。分別記錄正常和結(jié)扎后心電圖S-T段變化，測定血清乳酸脫氫酶（LDH）、肌酸激酶（CK）活性，計算心臟指數(shù)、左心室指數(shù)、左心室/心臟質(zhì)量比和心肌梗死率。結(jié)果 與模型組各時間點比較，丹參飲組在結(jié)扎后15~180 min心電圖S-T段均明顯降低，差異具有統(tǒng)計學意義（P<0.05）。與模型組比較，各組LDH、CK活性均明顯降低，差異具有統(tǒng)計學意義（P<0.05）；各組大鼠心臟指數(shù)、左心室指數(shù)、左心室/心臟質(zhì)量比均明顯降低，差異具有統(tǒng)計學意義（P<0.05）；各組心肌梗死質(zhì)量和梗死率均明顯降低，差異具有統(tǒng)計學意義（P<0.05）。結(jié)論 丹參飲對冠脈結(jié)扎大鼠心肌缺血具有保護作用，可能是增強冠脈供血和心肌代謝，改善心功能，有效抑制大鼠心肌缺血梗死。

Objective To evaluate the protective effect of Danshen Drink on myocardial ischemia in rats with coronary artery ligation, and study its mechanisms. Methods Sixty male Wistar rats were randomly divided into control group, model group, nifedipine group, Compound Danshen Tablets group, and Danshen Drink low, medium, and high dose group according to body weight, and each group had 10 rats. The dose of nifedipine was 0.167 mg/kg, the dose of Compound Danshen Tablets was 10 g/kg, and rats in these group were ig administer 10 mL/kg. Rats in Danshen Drink groups were ig administer Danshen Drink 0.5, 1.0, and 2.0 mL/kg (Equivalent to 0.27, 0.54, and 1.08 g/kg of raw materials). All the animals were treated for 7 d. Myocardial ischemia model of rats was established by ligating coronary artery after 7th day of administration of 30 min. The ST segment changes of ECG after normal and ligation were recorded, the activity of serum lactate dehydrogenase (LDH) and creatine kinase (CK) were determined, and cardiac index, left ventricular index, left ventricular/heart mass ratio and myocardial infarction were calculated. Results Compared with the time points of the model group, the ST segments of the 15-180 min electrocardiogram in the Danshen Drink group were significantly decreased after the ligation, and the difference was statistically significant (P<0.05). Compared with the model group, LDH and CK activity in each group was significantly decreased, and there was difference between two groups (P<0.05); the rats cardiac index, left ventricular index, left ventricular/heart mass ratio were significantly decreased, and there was difference between two groups (P<0.05); myocardial infarction quality and infarct rate were significantly decreased, and there was difference between two groups (P<0.05). Conclusion Danshen Drink has protective effect on myocardial ischemia in rats with coronary artery ligation, which may enhance coronary blood supply and myocardial metabolism, improve cardiac function, and effectively inhibit myocardial ischemia in rats.

四川省科技廳-瀘州市人民政府-瀘州醫(yī)學院聯(lián)合科研專項資金計劃項目（14ZC0040）