目的 建立HPLC梯度洗脫法測定復(fù)方鹿茸酒中尿囊素、山藥素I、淫羊藿苷、淫羊藿次苷I和寶藿苷I。方法 采用Agilent TC-C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相A：甲醇-乙腈（1：1）、流動(dòng)相B：水，梯度洗脫；0~22 min在224 nm波長下檢測尿囊素，22~45 min時(shí)在270 nm波長下檢測山藥素Ⅰ、淫羊藿苷、淫羊藿次苷Ⅰ和寶藿苷Ⅰ；體積流量0.9 mL/min；柱溫30℃；進(jìn)樣量10 μL。結(jié)果 尿囊素、山藥素I、淫羊藿苷、淫羊藿次苷I和寶藿苷I分別在7.98~159.60 μg/mL、4.75~95.00μg/mL、9.18~183.60 μg/mL、4.54~90.80μg/mL、4.92~98.40 μg/mL與峰面積線性關(guān)系良好；平均回收率分別為98.67%、96.86%、99.56%、98.15%、97.90%，RSD值分別為1.12%、0.85%、1.07%、1.49%、0.97%。結(jié)論 該方法簡便、準(zhǔn)確，重復(fù)性好，為進(jìn)一步完善復(fù)方鹿茸酒的質(zhì)量標(biāo)準(zhǔn)提供參考。

Objective To develop an HPLC gradient elution method for determination of allantoin, batatasin I, icariin, icarisid I and baohuoside I in Compound Lurong Vina. Methods HPLC method was adopted on Agilent TC-C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-acetonitrile (1:1)-water with gradient elution. The detection wavelengths were 224 nm in 0-22 min (determination of allantoin) and 270 nm in 22-45 min (determination of icariin, icarisid I, and baohuosideⅠ). The flow rate was 0.9 mL/min, and the column temperature was set at 30℃ with injection volume of 10 μL. Results Allantoin, batatasin I, icariin, icarisid I, and baohuoside I had good linearity in the ranges of 7.98-159.60 μg/mL, 4.75-95.00 μg/mL, 9.18-183.60 μg/mL¹, 4.54-90.80 μg/mL, and 4.92-98.40 μg/mL, respectively. The average recoveries were 98.67%, 96.86%, 99.56%, 98.15%, and 97.90%, with RSD of 1.12%, 0.85%, 1.07%, 1.49%, and 0.97%, respectively. Conclusions The method is simple, accurate and reproducible, and can provide a reference for the further improvement of the quality standard of Compound Lurong Vina.