目的 建立高效液相色譜梯度洗脫法同時測定心無憂片中洋川芎內(nèi)酯H、洋川芎內(nèi)酯I、洋川芎內(nèi)酯A、藁本內(nèi)酯、丹參素、丹酚酸B和丹參酮Ⅱ_A的方法。方法 采用Hydrosphere C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相A：甲醇–乙腈（2：1），流動相B：0.2%冰醋酸溶液，梯度洗脫（0~11 min，45.0% A；11~26 min，45.0%→68.0% A；26~39 min，68.0%→82.0% A；39~45 min，82.0%→45.0% A）；檢測波長：280 nm；體積流量：0.9 mL/min；柱溫：30℃；進樣量為10 μL。結(jié)果 洋川芎內(nèi)酯H、洋川芎內(nèi)酯I、洋川芎內(nèi)酯A、藁本內(nèi)酯、丹參素、丹酚酸B和丹參酮Ⅱ_A的線性范圍分別為2.08~41.60 μg/mL（r=0.999 3），3.36~67.20 μg/mL（r=0.999 8），4.29~85.80 μg/mL（r=0.999 9），6.26~125.20 μg/mL（r=0.999 6），3.49~69.80 μg/mL（r=0.999 2），49.08~981.60 μg/mL（r=0.999 7），7.17~143.40 μg/mL（r=0.999 5）；平均加樣回收率分別為96.81%、98.19%、99.24%、98.93%、97.39%、100.19%、98.63%，RSD值分別為1.51%、1.24%、0.98%、1.12%、0.84%、0.74%、1.44%。結(jié)論 建立的HPLC梯度洗脫法同時測定心無憂片中心無憂片中洋川芎內(nèi)酯H、洋川芎內(nèi)酯I、洋川芎內(nèi)酯A、藁本內(nèi)酯、丹參素、丹酚酸B和丹參酮Ⅱ_A7個成分的測定方法，供試品溶液處理簡便，溶液穩(wěn)定性好，檢測方法快捷、準確、靈敏度高，為心無憂片質(zhì)量標準的提高提供了參考。

Objective To develop HPLC gradient elution method for simultaneous determination of senkyunolide H, senkyunolide I, senkyunolide A, ligustilide, danshensu, salvianolic acid B, and tanshinoneⅡ_A in Xinwuyou Tablets. Methods The determination was carried out on Hydrosphere C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase was methanol-acetonitrile (2:1) and 0.2% acetic acid glacial solution with gradient elution. The elution procedures were as following:0 — 11 min with 45.0% A, 11 — 26 min with 45.0%→68.0% A, 26 — 39 min with 68.0%→82.0% A, and 39 — 45 min with 82.0%→45.0% A. The detection wavelengths were set at 280 nm. The flow rate was 0.9 mL/min, temperature of column was set at 30℃, and volume of injection was 10 μL. Results Senkyunolide H, senkyunolide I, senkyunolide A, ligustilide, danshensu, salvianolic acid B, and tanshinoneⅡ_Ahad good linearities in the range of 2.08 — 41.60 μg/mL (r=0.999 3), 3.36 — 67.20 μg/mL (r=0.999 8), 4.29 — 85.80 μg/mL (r=0.999 9), 6.26 — 125.20 μg/mL (r=0.999 6), 3.49 — 69.80 μg/mL (r=0.999 2), 49.08 — 981.60 μg/mL (r=0.999 7), and 7.17 — 143.40 μg/mL (r=0.999 5), respectively. The average recoveries were 96.81%, 98.19%, 99.24%, 98.93%, 97.39%, 100.19%, and 98.63%, respectively. And the corresponding RSD values were 1.51%, 1.24%, 0.98%, 1.12%, 0.84%, 0.74%, and 1.44%, respectively. Conclusion The established method of HPLC gradient elution method for simultaneous determination of senkyunolide H, senkyunolide I, senkyunolide A, ligustilide, danshensu, salvianolic acid B, and tanshinone Ⅱ_A in Xinwuyou Tablets is simple for the solution treatment, stable for the solution, and fast for the detection method. The method is accurate and sensitive which can be applied to reference for the quality control of Xinwuyou Tablets.