目的 研究桂枝或炙甘草劑量變化對(duì)桂枝甘草湯藥效組分的影響。方法 采用HPLC-DAD多波長(zhǎng)切換法同時(shí)測(cè)定10種成分的變化。Hypersil Gold-C₁₈色譜柱（250 mm×4.6 mm，5μm）；流動(dòng)相乙腈-0.1%磷酸溶液，梯度洗脫；檢測(cè)波長(zhǎng)230 nm（苯甲酸鈉、甘草苷、甘草素）、254 nm（桂皮醇、甘草酸）、276 nm（香豆素、桂皮酸、桂皮醛）、370 nm（異甘草苷、異甘草素）；體積流量0.8 mL/min；柱溫20℃；進(jìn)樣量20 μL?？疾旃鹬蛑烁什輨┝孔兓瘜?duì)各藥材、桂枝甘草湯藥效組分的影響。結(jié)果 適當(dāng)增加桂枝（炙甘草）劑量可促進(jìn)炙甘草（桂枝）藥效組分的溶出，當(dāng)桂枝（炙甘草）超過一定劑量，炙甘草（桂枝）藥效組分的含量反而降低。桂枝、炙甘草劑量變化時(shí)其自身的藥效組分含量變化明顯。隨著桂枝或炙甘草劑量的增加，桂枝（炙甘草）5種藥效組分總量均呈先增后減的趨勢(shì)，當(dāng)配伍劑量與經(jīng)方相一致時(shí)有最大值。結(jié)論 桂枝（炙甘草）劑量變化對(duì)炙甘草（桂枝）藥效組分、自身藥效組分及藥效組分總量均有影響，提示桂枝甘草湯用藥劑量科學(xué)合理。

Objective To study the influence of Cinnamomi Ramulus and honey-fired Glycyrrhizae Radix dose changes on active components alignments in Guizhi Gancao Decoction. Methods Ten components were simultaneously determined by HPLC-DAD with changing ultraviolet-visible wavelength method. Hypersil Gold-C₁₈ (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution, with a flow rate of 0.8 mL/min. The column temperature was set at 20℃, and injection volume was 20 μL. The detection wavelength was set at 230 nm (sodium benzoata, liquiritin, and linquiritigenin), 254 nm (cinnamic alcohol and glycyrrhizic acid), 276 nm (coumarin, cinnamic acid, and cinnamaldehyde), and 370 nm (isoliquiritin and isolinquiritigenin). Dose changes of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix on active components in Chinese herbal medicine in prescription and Guizhi Gancao Decoction were investigated.Results The dosage of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix could promote the dissolution of the active components. When Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix exceeded a certain dose, the contents of active components were decreased. When dosages of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix changed, their active components had obvious changes. With the increase of the dose of Cinnamomi Ramulus and honey-fired Glycyrrhizae Radix, the total amounts of the five active components increased first and then decreased, and when the compatibility dose was the same as that of Guizhi Gancao Decoction prescription, they reached the maximum. Conclusion The dose change of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix has influence on the active components, itself active components, and total active components, and it indicates that the dosage of Guizhi Gancao Decoction is scientific and reasonable.