目的 建立HPLC法測定不同產(chǎn)地刺五加中原兒茶酸、紫丁香苷、綠原酸、刺五加苷E和異嗪皮啶。方法 采用Welch Materials C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相為乙腈-0.1%磷酸水，梯度洗脫；體積流量為1.0 mL/min；柱溫30℃；檢測波長214 nm；進樣體積10 μL。測定結(jié)果采用主成分分析法進行分析。結(jié)果 5種成分在測定質(zhì)量濃度范圍內(nèi)線性關(guān)系良好，r均大于0.999 9；平均回收率為99.66%~101.25%，RSD值為1.92%~3.03%；不同產(chǎn)地的11批刺五加中原兒茶酸、紫丁香苷、綠原酸、刺五加苷E和異嗪皮啶的質(zhì)量分?jǐn)?shù)范圍分別為0.004%~0.016%、0.080%~0.152%、0.372%~0.806%、0.057%~0.103%、0.002%~0.010%。主分成分析結(jié)果顯示，黑龍江產(chǎn)刺五加藥材質(zhì)量較佳。結(jié)論 該方法快速、簡便、準(zhǔn)確，多成分測定的質(zhì)量評價模式可用于刺五加藥材的質(zhì)量控制。

Objective To establish an HPLC method for simultaneous determination of protocatechuic acid, syringing, chlorogenic acid, eleutheroside E and isofraxidin in Acanthopanax senticosus (Rupr. et Maxim.) Harms from different habitats. Methods Separation was carried out on Welch Materials C₁₈ column (250 mm×4.6 μm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid water with gradient elution. The detection wavelengths were set at 214 nm. The flow rate was 1.0 mL/min, temperature of column was set at 30℃, and volume of injection was 10 μL. The result was analyzed by PCA. Results The linear relations of five active components in the range of mass concentration was good with perfect precision with r all more than 0.999 9. The average recovery rates were 99.66%-101.25%, and RSD was 1.92%-3.03%. The contents in 11 batch of the A. senticosus from different habitats were calculated, and the results were as following:protocatechuic acid was about 0.004%-0.016%, syringing was 0.080%-0.152%, chlorogenic acid was 0.372%-0.806%, eleutheroside E was 0.057%-0.103%, and isofraxidin was 0.002%-0.010%. Conclusion The method is rapid, convenient, and with precision and accuracy, and the multi-component determination method is suitable for the quality control of A. senticosus.

天津市科技計劃項目（12ZCDZSY11600； 14ZCZDSY00005）；天津市科技支撐計劃項目（15ZCZDSY00470）；天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計劃（14JCQNJC13800； 15JCYBJC29500）