目的 研究潰瘍性結(jié)腸炎患者腸道緊密連接蛋白o(hù)ccludin、ZO-1的表達(dá)及其臨床意義。方法 收集2014年1月-2017年10月在中國醫(yī)科大學(xué)附屬盛京醫(yī)院住院的潰瘍性結(jié)腸炎患者臨床資料和組織標(biāo)本作為觀察組，共80例，手術(shù)切除后經(jīng)病理學(xué)證實的正常斷端結(jié)腸組織20例，作為對照組。觀察組按照Mayo臨床評分（MCS）分為臨床緩解組、輕度活動期組、中度活動期組、重度活動期組。免疫組織化學(xué)法檢測腸道緊密連接蛋白o(hù)ccludin、ZO-1表達(dá)情況，并分析其與臨床參數(shù)的相關(guān)性。結(jié)果 MCS和Mayo內(nèi)鏡評分（MCSe）在臨床緩解組、輕度活動期組、中度活動期組、重度活動期組呈上升趨勢，各組間差異均具有統(tǒng)計學(xué)意義（P<0.05）。與對照組比較，各觀察組均顯著升高（P<0.05），Geboes指數(shù)在各組中呈上升趨勢，各組間差異均具有統(tǒng)計學(xué)意義（P<0.05）。與對照組比較，緊密連接蛋白（occludin和ZO-1）在觀察組中的表達(dá)下降。ZO-1、occludin的表達(dá)與MCS、MCSe、Geboes指數(shù)、C-反應(yīng)蛋白（CRP）呈負(fù)相關(guān)。結(jié)論 緊密連接蛋白ZO-1、occludin可能會成為評估潰瘍性結(jié)腸炎患者腸道炎癥程度和預(yù)測黏膜愈合的指標(biāo)。

Objective To study expression and clinical significance of intestinal tight junction (TJ) protein occludin and ZO-1 in patients with ulcerative colitis (UC). Methods The clinical data and colonic biopsies of UC in Shengjing Hospital of China Medical University from January 2014 to October 2017 were collected as the experimental group, and 80 cases were treated by pathology, and 20 cases of normal distal colon after resection were confirmed as the control group. The experimental group was divided into clinical remission group, mild activity group, moderate activity group, and severe activity stage group according to Mayo clinical score (MCS). The expressions of TJ proteins which included occluding and ZO-1 were assessed by immunohistochemistry, and its correlation with clinical characteristics were also analyzed. Results MCS and Mayo endoscopic subscore (MES) showed an upward trend in the clinical remission group, the mild activity period group, the moderate activity stage group, and the severe active stage group, and the differences were statistically significant (P<0.05). Compared with the control group, all experimental groups were significantly decreased (P<0.05), and the Geboes indexes in the remission group, the mild active period group, the moderate active stage group, and the severe active stage group were increased, and the differences were statistically significant (P<0.05). Compared with the control group, the expressions of TJ protein (occludin and ZO-1) were increased in the experimental group. The expressions of ZO-1 and occludin were negatively correlated with MCS, MCSe, Geboes index, and C-reactive protein (CRP). Conclusion TJ proteins (Zo-1 and occludin) might be an potential indicator of intestinal inflammation and prognosis of UC.

遼寧省自然科學(xué)基金資助項目（20170540529）